Evaluación ginecológica y niveles de P4 en vacas receptoras pós-ovulación, sincronizadas con eCG en diferentes momentos
The objective of this research was to evaluate the effect of Equine Chorionic Gonadotropin (eCG) application at different times within a synchronization protocol on the formation of corpora lutea and progesterone (P4) levels in recipient cows. Twenty Bos taurus crossbred bovine cows, with a body con...
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| Format: | masterThesis |
| Language: | spa |
| Published: |
2025
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| Subjects: | |
| Online Access: | https://dspace.unl.edu.ec/jspui/handle/123456789/32384 |
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Add Tag | Summary: | The objective of this research was to evaluate the effect of Equine Chorionic Gonadotropin (eCG) application at different times within a synchronization protocol on the formation of corpora lutea and progesterone (P4) levels in recipient cows. Twenty Bos taurus crossbred bovine cows, with a body condition ≥ 2.5, between 2 and 3 calvings, and fed a similar diet based on gramalote grass (Axonopus scoparius) and concentrates were used. Cows were divided into two treatments: T1 (day 0: BE (estradiol benzoate) + CIDR (progesterone in intravaginal device) + PGF2α (prostaglandin); day 6: 800 U. I of eCG (Equine Chorionic Gonadotropin); day 8: removal of CIDR + PGF2α; day 9: BE; day 10: artificial insemination (IATF) (NOT PERFORMED) + GNRH and T2 (day 0: BE + CIDR + PGF2α; day 8: 800 U.I of eCG, removal of CIDR + PGF2α; day 9: BE; day 10: IATF) (NOT PERFORMED) + GNRH. The variables analyzed were: number of corpus luteum (CL), diameter of CL, uterine tone, echogenicity of the uterus, presence of intrauterine fluid and progesterone concentration at day 7 post-ovulation. The size of the corpus luteum at 7 days post-ovulation was 26.53 ± 1.84 mm for T1 and 28.77 ± 1.84 mm for T2, without significant differences (P>0.05) as well as uterine tone, uterine echogenicity, presence of intrauterine fluid. P4 concentration also showed no significant differences between treatments: 3.61 ng-mL-1 for T1 (eCG day 6) and 4.50 ng-mL-1 for T2 (eCG day 8) and the number of corpora lutea at 7 days post-ovulation. In conclusion, there was no significant effect of the time of application of Equine Chorionic Gonadotropin (eCG) (day 6 or day 8) on corpus luteum characterization and plasma progesterone concentration (P4). |
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