Procesos biotecnológicos para la inducción in vitro de raíces a partir de explantes obtenidos de vitroplantas de cinchona officinalis l., provenientes de relictos boscosos de la provincia de Loja
Cinchona officinalis L., known as the National Tree of Ecuador due to its medicinal properties in the fight against malaria, presents a deficient natural regeneration, which has not been able to be compensated using in vivo propagation techniques. For this reason, the present research aims to contri...
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| Format: | bachelorThesis |
| Jezik: | spa |
| Izdano: |
2020
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| Online dostop: | https://dspace.unl.edu.ec/jspui/handle/123456789/23716 |
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Označite | Izvleček: | Cinchona officinalis L., known as the National Tree of Ecuador due to its medicinal properties in the fight against malaria, presents a deficient natural regeneration, which has not been able to be compensated using in vivo propagation techniques. For this reason, the present research aims to contribute to the definition of a methodology to root explants of C. officinalis. For it, two trials with different hormonal concentrations were installed, in the first one an auxin hormonal balance (AIB + 2,4-D) was used, while, in the second, the auxin-cytokinin hormonal balance (AIB + BAP) was used, and the following variables were evaluated: contamination, survival, phenolic oxidation; number of leaves, shoots and nodes; height, days to rooting, percentage of rooting, number and length of roots per explant. In rooting, the hormonal concentration (0,5 mg L-1 IBA + 0 mg L-1 BAP), corresponding to the T2 treatment of the second objective, turned out to be the most effective, since it presented 70 % rooting, obtained 4,67 roots per explant, and reached 1,2 mm in root length. Consequently, the hormonal concentration (1,0 mg L-1 AIB + 1,0 mg L-1 BAP), belonging to the T6 treatment of the auxin-cytokinin hormonal balance, obtained 13,37 average nodes per explant and formed 21,27 average leaves per explant. Similarly, the T5 treatment (1,0 mg L-1 AIB + 0,5 mg L-1 2,4-D), corresponding to the auxin hormonal balance, reached the highest height per explant, with a value of 4,72 cm. In conclusion, to induce rooting, auxins were more effective than the auxin-cytokinin interaction, which acted positively in the formation of knots, shoots and leaves. Finally, it is necessary to test and find an appropriate hormonal balance for the formation of roots in the explants of C. officinalis. Key words: Cinchona officinalis L., induction, rooting, explants, hormonal balance |
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