Prevalencia molecular de Babesia bovis y Babesia bigemina en explotaciones ganaderas del sector sur de la provincia de Zamora Chinchipe, Ecuador.
A total of 96 and 70 samples of bovine blood taken from asymptomatic animals in the south sector of the province of Zamora Chinchipe were analyzed using the Giemsa and nPCR techniques, respectively, in order to make a diagnosis of Babesia spp. through the Giemsa technique, it was reported a general...
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| Format: | bachelorThesis |
| Language: | spa |
| Published: |
2017
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| Online Access: | http://dspace.unl.edu.ec/jspui/handle/123456789/18460 |
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| Summary: | A total of 96 and 70 samples of bovine blood taken from asymptomatic animals in the south sector of the province of Zamora Chinchipe were analyzed using the Giemsa and nPCR techniques, respectively, in order to make a diagnosis of Babesia spp. through the Giemsa technique, it was reported a general prevalence of 32,29 % and by the nPCR technique an overall prevalence of 71,43% of B. bovis 44 % and of B. bigemina 50% . In terms of age and according to the two techniques, the animals who were less than one year reported positive results in 7 % and 50 % respectively; The group between one and two years old was positive in a 27 % and 86 %; Animals aged between three and four years, in a 33 % and 60 %; While the group of animals older than four years tested positive in 47 % and 73 %. In relation to the breeds it is observed that the Mestizo breed had a prevalence of 18% and 78%, the Brahman breed with 40% and 75%, the Brown swiss breed with 36% and 64%, the Holstein breed with 0% and 63% % This for Giemsa and nPCR respectively. We conclude that the nPCR technique for its sensitivity and specificity is much more effective than the Giemsa technique in the diagnosis of asymptomatic animals; With which we can assert that the prevalence of Babesia spp. In the province of Zamora Chinchipe, with a greater presence of B. bovis. bigemina. |
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