Evaluación de protocolos de transporte de semen bovino congelado bajo diferentes temperaturas y tiempos de descongelación
The present work was carried out at the Animal Reproduction Laboratory of the Universidad Nacional de Loja, with the objective of comparing temperature protocols for transporting straws, analyzing motility and viability at different time intervals. The first protocol consisted of transporting them a...
Sábháilte in:
| Príomhchruthaitheoir: | |
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| Formáid: | masterThesis |
| Teanga: | spa |
| Foilsithe / Cruthaithe: |
2025
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| Ábhair: | |
| Rochtain ar líne: | https://dspace.unl.edu.ec/jspui/handle/123456789/32095 |
| Clibeanna: |
Cuir clib leis
Níl clibeanna ann, Bí ar an gcéad duine le clib a chur leis an taifead seo!
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Cuir clib leis | Achoimre: | The present work was carried out at the Animal Reproduction Laboratory of the Universidad Nacional de Loja, with the objective of comparing temperature protocols for transporting straws, analyzing motility and viability at different time intervals. The first protocol consisted of transporting them at a temperature of 5°C, the second protocol at 39°C, and the third protocol at 42°C. Nine straws per protocol were evaluated, and each protocol was analyzed at three time intervals, at 30, 60 and 90 minutes. The variables analyzed were: individual motility, percentage of live spermatozoa, percentage of dead spermatozoa. The type of experimental design used was a completely randomized design with a 3x3 factorial arrangement. According to the analysis of variance of the three study variables, there is no effect of temperature, time or the interaction of these two factors on sperm motility, the proportion of live spermatozoa and the proportion of dead spermatozoa. However, the results showed that the best transport protocol is in a medium with a temperature of 39 degrees Celsius, since the average individual motility was 48.69% and the time interval with the best values in individual motility was 30 minutes, since an average of 50.82% was achieved. With respect to viability, the best average of live spermatozoa was recorded at a temperature of 39 degrees Celsius (50.56%), while the time interval with the best average of live spermatozoa was 30 minutes (54.44%). In conlusion, the transport protocol at 39°C is the most suitable for preserving the quality of bovine semen, as it ensures higher individual motility and a greater proportion of live sperm, especially in the first 30 minutes post-thawing. |
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