Efecto citotóxico del extracto acuoso de las hojas de Annona Cherimola en una línea celular de cáncer de pulmón en medio cultivo con pH ácido

The Acetogenins which belong to the genre Annona in several intra-laboratory tests have shown their cytotoxic activity against tumor cells; therefore we have developed the following investigation with an experimental-perspective design guided to evaluate the cytotoxic effect of aqueous extracts from the Annona cherimola leaves in the line cell of lung cancer with a medium crop of Ph. acid, its focused to determine the grade/level of proliferation and the viability of A-549 cells versus the extract of the proliferation cell technique which consists on assessing the cell development meanwhile its viability (living-dead) was determined through blue staining tripán every 6 hours during 4 days. In order to conclude this study we used a positive control (cells a-549 in front of cisplatin) and a negative control (cells A-549 in front of the medium crop Roswell Park Memorial Institute (R.P.M.I.) completed) in order to control the quality of the study. The proliferation cell degree in A-549 versus the extract aqueous from the Annona ch. leaves in different concentrations (concentrated, 1:10-1:50) in 6 hours’ time gives a 0% of confluence meanwhile in 36 hours of different concentration we can clearly obtain a 74,4%, 71,9% y 80,5% ; nevertheless at 72 hours his development is diminished acquiring the following values 68,2 %, 61,1% and a 64,7%; meanwhile in order to assess its cell viability an 82,2% of cytotoxicity was acquired with the medicine, and a 34,4% in extract while normal cells, versus the diluted 1:10 extract from the Annona ch. leaves develop a 10,9% during the first 72 hours of incubation. Finally we can conclude the level of cytotoxicity from the extract from the Annona ch. leaves versus the cells A-549 and Mo nuclear is very low in relation to the cisplatin which causes a high cytotoxicity. Key Words: - Annona Cherimola, Acetogenins, Cytotoxicity, pH acid, Lung cancer, Proliferation Cellular and Viability Cellular.

• 主要作者: Medina Lanche, Mayra Alexandra (author)
• 格式: bachelorThesis
• 語言: spa
• 出版: 2015
• 主題: MEDICINA TRADICIONA; PLANTAS MEDICINALES; CÁNCER DE PULMÓN
• 在線閱讀: http://dspace.unl.edu.ec/jspui/handle/123456789/13745