Identificación molecular de microorganismos rizosféricos provenientes de la provincia de Loja para la formulación de un bioinoculante agrícola
The excessive use of chemical fertilizers has caused disturbances in the agroecosystem, leading to an ecological imbalance. For that reason, the integration of bioinoculants is suggested as an accessible alternative, as they help improve nutrient use, soil health, and overcome some of the limitation...
Αποθηκεύτηκε σε:
| Κύριος συγγραφέας: | |
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| Μορφή: | bachelorThesis |
| Γλώσσα: | spa |
| Έκδοση: |
2024
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| Θέματα: | |
| Διαθέσιμο Online: | https://dspace.unl.edu.ec/jspui/handle/123456789/29858 |
| Ετικέτες: |
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Προσθήκη ετικέτας | Περίληψη: | The excessive use of chemical fertilizers has caused disturbances in the agroecosystem, leading to an ecological imbalance. For that reason, the integration of bioinoculants is suggested as an accessible alternative, as they help improve nutrient use, soil health, and overcome some of the limitations associated with fertilizer use. The objective of this research was to molecularly identify rhizospheric microorganisms from the province of Loja to formulate an agricultural bioinoculant and thus provide a sustainable biotechnological alternative to enhance production. DNA extraction was performed on previously isolated strains (Azotobacter, Azospirillum, and Pseudomonas), which were molecularly identified at the Biosequence company using the amplicon metagenomics process for the respective bioinformatic analysis, where the genders were determined but not the respective species and families. For the formulation, the necessary components for proper growth were established, and the bacteria of interest were seeded in a total of four formulas, also pH was measured, the growth kinetics of each bacterium in its formulation were evaluated, and it was determined that the pH of the medium is suitable within a range of 7.0 - 7,20 and the treatments that show significant differences are: in Azospirillum, formula three, with an initial concentration of 4,8E+09 CFU/ml and optimal growth up to 48 hours with a total of 1,5E+10 CFU/ml, followed by a decrease towards 72 hours; in the case of Azotobacter, the best treatment is formula four, with an initial concentration of 3,5E+09 CFU/ml and exponential growth up to 72 hours with a total of 1,5E+10 CFU/ml. In the case of the Pseudomonas genus, no treatment is statistically significant. In addition, antagonism between the tests was analyzed, determining that there is no competition. |
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