Consorcios bacterianos asociados a los escarabajos estercoleros (Coleoptera: Scarabaeinae) y su relación con la estructura del suelo en la Estación Experimental el Padmi

Bacterial communities are key in the functioning of the ecosystem, considering them essential drivers of biogeochemical cycles. The following study characterized bacterial consortia associated with dung beetles (Coleoptera: Scarabaeinae) and soil in three areas (conserved, restored, and grassland) o...

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Gorde:
Xehetasun bibliografikoak
Egile nagusia: Salazar Romero, Soraya Lizbeth (author)
Formatua: bachelorThesis
Hizkuntza:spa
Argitaratua: 2023
Gaiak:
Sarrera elektronikoa:https://dspace.unl.edu.ec/jspui/handle/123456789/26480
Etiketak: Etiketa erantsi
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Deskribapena
Gaia:Bacterial communities are key in the functioning of the ecosystem, considering them essential drivers of biogeochemical cycles. The following study characterized bacterial consortia associated with dung beetles (Coleoptera: Scarabaeinae) and soil in three areas (conserved, restored, and grassland) of the El Padmi Experimental Station. Bacteria were isolated from the three representative soil samples of each plant cover and from the 6 beetle specimens collected using culture-dependent methods: Nutrient Agar (AN) and Trypticase-Soy Agar (TSA). For the morphological identification of the isolated bacteria, the Gram stain method was used and it was associated with bacterial genera according to the colonial and cellular morphological characteristics. The isolation was effective in soils and beetles, so obtaining 29 pure cultures for soils, being the Natural Forest the most representative with 12 pure cultures and 54 pure cultures from the intestine of beetles, being the Ribera Forest the most representative with 19 pure cultures. According to the morphological results obtained, the possible assignment at the level of bacterial genus was made using the Manuel de Brenner. Being Streptococcus, Staphylococcus and Bacillus, the genera present in all soil samples; and the bacteria found in the intestine of the observed species Dichotomius quinquelobatus, Coprophanaeus telamon and Eurysternus caribaeus, were related to the genera: Bacillus, Clostridium, Acinetobacter and Enterobacter. Thus, evidencing that the pattern of concurrence of certain genera is of fundamental ecological importance within the ecosystem. Keywords: Bacterial communities, culture media, isolation, morphological analysis, bacterial genera.