Comparación de comunidades bacterianas en ecosistemas contrastantes de cuencas hidrográficas en los cantones Puyango y Puyo, Ecuador

This study determined the composition of bacterial communities in two contrasting ecosystems of Ecuador, located in the Puyango basin located to the south of Ecuador representing a degraded ecosystem, and the Puyo basin within the UEA in the Amazon, which presents Characteristic of being an ecosystem in a state of conservation. For this reason, the objective was to characterize and compare bacterial communities in water and sediment samples, presenting differences in the microorganisms found at each sampling site, allowing the evaluation of their natural state. The results obtained allowed to show the growth kinetics of the colony forming units (UFC), which in both ecosystems showed an asynchronous growth due to the difference in the cell cycle, establishing its growth phase within 24 and 72 hours, thus determining The greater composition of the bacterial community and its greater variability present in sediment samples, in both study areas, in addition their morphological characteristics allowed to identify the type of microorganism, presented mostly Gram-positive Bacillus microorganisms, and their unique characteristics identified members Of genus Bacillus, Coco, Micrococo, Spirillum and Sarcinas. Subsequently the amplification of the genes of the subregion 16S rRN was achieved. However, it was not possible to identify the species of each isolate due to the lack of inputs for molecular sequencing in DNA sequencing.

• المؤلف الرئيسي: Maita Fernández, Lourdes Tamara (author)
• التنسيق: bachelorThesis
• اللغة: spa
• منشور في: 2017
• الموضوعات: CUENCAS HIDROGRÁFICAS; ECOSISTEMAS
• الوصول للمادة أونلاين: http://dspace.unl.edu.ec/jspui/handle/123456789/18527