Efecto insecticida de eugenol purificado en el control del gorgojo callosobruchus maculatus en fríjol del género vigna

The research denominated "INSECTICIDE EFFECT OF PURIFIED EUGENOL IN THE CONTROL OF GORGOJO Callosobruchus maculatus IN FRÍJOL DEL GÉNERO Vigna". The present work had as objectives to evaluate the susceptibility of C. maculatus exposed to pure eugenol via fumigant; To determine the instantaneous rate of growth and development period of C. maculatus exposed to sub-lethal concentrations of eugenol; To discover the adult eugenol repellent capacity of C. maculatus; Calculate the mass loss of bean grains treated with sub-lethal doses of eugenol and infested with adults of C. maculatus. In the methodology, the scientific experimental, statistical method and the technique direct observation was used to elaborate each objective. Eugenol toxicity was evaluated through preliminary tests to determine the lethal concentration for 50 and 95% of mortality (LC50 and LC95) of adult insects. The tests were conducted in flasks (800 ml capacity) with 50 grams of cowpea beans, where they were introduced 10 couples C. maculatus (adults and <48 hours old) and exposed to different concentrations. To avoid direct contact of eugenol with insects or beans, using cotton thread small plates of filter paper containing the eugenol were suspended from the inside of the lid of each jar, five replicates per treatment were performed and mortality it was counted after 24 hours. The instantaneous growth rate (ri) was calculated from the CL20, CL40, CL60, CL80, CL100, with the testes (exposure) similar to those of toxicity, ri was obtained with the total number of insects emerged after 46 days. Similar procedure was performed for the period of development, with the difference that every two days (after first emerged insect) left counting the number of emerged insects. Repellency was made with treated bean with CL50 and CL100 for 24 hours , then the bean was placed in arenas with four arms and a box at each end of the arm (2 arms for bean untreated and 2 for treated bean) and then 24 hours the number of insects in each box was recorded. Mass loss was calculated from the final weight of the grains of each treatment and the initial weight of these same beads. The results show that LC50 is 24.80 uL L-1 and the LC95 is 81.14 uL L-1. In addition it was found that the ri decreased as the concentration was increased, achieving stability in the CL40. The development period was also affected by the lethal concentrations of eugenol, two days delayed the start of the emergency for different treatments compared to control. Also was observed repellent effect of eugenol in CL100 (134.13 uL L-1) while the CL50 (24.80 uL L-1) eugenol was attractive effect for this insect. The mass loss of cowpea was proportional to the number of emerged insects. According to these results it is concluded that eugenol has insecticidal activity to C. maculatus via fumigant, may be an alternative for controlling of beans this pest in small storage units of the genus. As a recommendation it is extremely important to use organic products in order to avoid damage to the health of farmers, the environment and also to avoid massive losses of stored grain harvest

• 1. Verfasser: González Armijos, María José (author)
• Format: bachelorThesis
• Sprache: spa
• Veröffentlicht: 2016
• Schlagworte: INGENIERA ADMINISTRACIÒN; PRODUCCIÒN AGROPECUARIA; EFECTO INSECTICIDA; EUGENOL PURIFICADO
• Online Zugang: http://dspace.unl.edu.ec/jspui/handle/123456789/17592