Aislamiento y caracterización morfológica, bioquímica y fisiológica de microorganismos rizosféricos provenientes de suelos agrícolas de la provincia de Loja
Beneficial rhizospheric microorganisms are noted for increasing nutrient availability, producing hormones and protecting plants against biotic and abiotic stresses. The objective of this work was to characterize rhizospheric microorganisms isolated from agricultural soils of the province of Loja in...
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| Médium: | bachelorThesis |
| Jazyk: | spa |
| Vydáno: |
2022
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| Témata: | |
| On-line přístup: | https://dspace.unl.edu.ec/jspui/handle/123456789/25655 |
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Přidat tag | Shrnutí: | Beneficial rhizospheric microorganisms are noted for increasing nutrient availability, producing hormones and protecting plants against biotic and abiotic stresses. The objective of this work was to characterize rhizospheric microorganisms isolated from agricultural soils of the province of Loja in order to select promising strains of interest as bioinoculants. Soil samples of horticultural crops (1 kg per site) were collected from the most representative areas of the Zapotillo, Macará, Saraguro and Loja cantons by simple random sampling and then transported to the Biotechnology Center of the National University of Loja. For the isolation of rhizospheric microorganisms, serial dilutions of each sample of up to 10- 3 and 10- 4 were made and sown in triplicate in Petri dishes with Nutrient Agar for bacteria and Rose Bengal for fungi, incubated at 26 °C and 28 °C for 48 and 72 hours respectively, then, the microorganisms that presented morpho-cultural characteristics of interest were purified. After the determined incubation period and in relation to the results of the growth kinetics, the bacterial isolates were preserved in glycerol (20 % and 100 %) and the fungal isolates in sterile distilled water for subsequent morphological, biochemical and physiological characterization. In all cases, the rhizospheric isolates showed interesting characteristics such as motility, positive catalase, phosphate solubilization, cellulolytic activity, siderophore production, biological nitrogen fixation and indole-3-acetic acid production, with the MA3 strain from the Macará canton standing out. Therefore, these promising results lay the groundwork for the selection of strains with the ability to promote plant growth that could be an alternative to chemical fertilization in cultivars of agricultural interest. Key words: Rhizospheric microorganisms, growth kinetics, cryopreservation, motility, phosphate solubilization, siderophore production, Azotobacter, Azospirillum, Pseudomonas and Penicillium sp. |
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