Evaluación de dos protocolos de sincronización de celo en cuyes (Cavia porcellus)

The objective of the study was to establish measures of the reproductive system, observe epithelial-vaginal cells and verify the efficiency of synchronization protocols with reduction in application times. Three experiments were conducted. Experiment 1 identified measurements of ovaries, oviducts, h...

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Հիմնական հեղինակ: Domínguez Pinta, Luis Fernando (author)
Ձևաչափ: bachelorThesis
Լեզու:spa
Հրապարակվել է: 2023
Խորագրեր:
Առցանց հասանելիություն:https://dspace.unl.edu.ec/jspui/handle/123456789/26745
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author Domínguez Pinta, Luis Fernando
author_facet Domínguez Pinta, Luis Fernando
author_role author
collection Repositorio Universidad Nacional de Loja
dc.contributor.none.fl_str_mv Serrano Recalde, Elena Carolina
dc.creator.none.fl_str_mv Domínguez Pinta, Luis Fernando
dc.date.none.fl_str_mv 2023-04-11T15:35:12Z
2023-04-11T15:35:12Z
2023-04-11
dc.format.none.fl_str_mv 66 p.
application/pdf
dc.identifier.none.fl_str_mv https://dspace.unl.edu.ec/jspui/handle/123456789/26745
dc.language.none.fl_str_mv spa
dc.publisher.none.fl_str_mv Loja
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/ec/
info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv reponame:Repositorio Universidad Nacional de Loja
instname:Universidad Nacional de Loja
instacron:UNL
dc.subject.none.fl_str_mv OVSYNCH
COBAYA
MEMBRANA VAGINAL
dc.title.none.fl_str_mv Evaluación de dos protocolos de sincronización de celo en cuyes (Cavia porcellus)
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/bachelorThesis
description The objective of the study was to establish measures of the reproductive system, observe epithelial-vaginal cells and verify the efficiency of synchronization protocols with reduction in application times. Three experiments were conducted. Experiment 1 identified measurements of ovaries, oviducts, horns and uterine body, cervix and vaginal canal, in addition to the weight of the ovaries of 56 guinea pigs aged 8, 9, 10 and 12 weeks; in experiment two in 69 guinea pigs the vaginal membrane was observed and cytology was used to count and classify the cells of the vaginal epithelium; in experiment three two protocols were applied, Ovsynch and Ovsynch + Progesterone (O+P4); the same analysis of experiment 2 was performed and ultrasound was used for diagnosis of pregnancy. For the results obtained, descriptive statistics and analysis of ANOVA variance and Chi-square test (X2) were performed. In Experiment one: age influenced absolute measurements of ovaries, uterine horns, cervix and vaginal canal being greater (p≤0.05) in guinea pigs of 12 weeks; relative measurements were greater (p≤0.05) in most guinea pig structures of 8 weeks. Experiment two: there was dependence (p<0.001) of the vaginal membrane with the type of cells observed. Experiment three: both protocols showed a higher percentage of parabasal cells (47.85±21.22% and 41.23±24.22%); there was dependence (p<0.001) between the vaginal membrane, cell type and protocol application. The pregnancy percentage showed no difference (p>0.05) between treatments. It is concluded that the age and weights of guinea pigs influenced the absolute and relative measures of the reproductive organs; the vaginal membrane is related to the type of vaginal epithelial cells and the protocols of synchronization, which, despite not being efficient to present oestrus, influenced the presentation of the type of cells and vaginal membrane. Keywords: Ovsynch, guinea pig, oestrus, uterus, vaginal membrane.
eu_rights_str_mv openAccess
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publisher.none.fl_str_mv Loja
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repository.name.fl_str_mv Repositorio Universidad Nacional de Loja - Universidad Nacional de Loja
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spelling Evaluación de dos protocolos de sincronización de celo en cuyes (Cavia porcellus)Domínguez Pinta, Luis FernandoOVSYNCHCOBAYAMEMBRANA VAGINALThe objective of the study was to establish measures of the reproductive system, observe epithelial-vaginal cells and verify the efficiency of synchronization protocols with reduction in application times. Three experiments were conducted. Experiment 1 identified measurements of ovaries, oviducts, horns and uterine body, cervix and vaginal canal, in addition to the weight of the ovaries of 56 guinea pigs aged 8, 9, 10 and 12 weeks; in experiment two in 69 guinea pigs the vaginal membrane was observed and cytology was used to count and classify the cells of the vaginal epithelium; in experiment three two protocols were applied, Ovsynch and Ovsynch + Progesterone (O+P4); the same analysis of experiment 2 was performed and ultrasound was used for diagnosis of pregnancy. For the results obtained, descriptive statistics and analysis of ANOVA variance and Chi-square test (X2) were performed. In Experiment one: age influenced absolute measurements of ovaries, uterine horns, cervix and vaginal canal being greater (p≤0.05) in guinea pigs of 12 weeks; relative measurements were greater (p≤0.05) in most guinea pig structures of 8 weeks. Experiment two: there was dependence (p<0.001) of the vaginal membrane with the type of cells observed. Experiment three: both protocols showed a higher percentage of parabasal cells (47.85±21.22% and 41.23±24.22%); there was dependence (p<0.001) between the vaginal membrane, cell type and protocol application. The pregnancy percentage showed no difference (p>0.05) between treatments. It is concluded that the age and weights of guinea pigs influenced the absolute and relative measures of the reproductive organs; the vaginal membrane is related to the type of vaginal epithelial cells and the protocols of synchronization, which, despite not being efficient to present oestrus, influenced the presentation of the type of cells and vaginal membrane. Keywords: Ovsynch, guinea pig, oestrus, uterus, vaginal membrane.El objetivo del estudio fue establecer medidas del aparato reproductor, observar células epitelio-vaginales y verificar la eficiencia de protocolos de sincronización con reducción en tiempos de aplicación. Se desarrolló tres experimentos. En el experimento uno se determinó medidas de ovarios, oviductos, cuernos y cuerpo uterino, cérvix y conducto vaginal, además del peso de ovarios de 56 cobayas de 8, 9, 10 y 12 semanas de edad; en el experimento dos en 69 cobayas se observó la membrana vaginal y se empleó citología para contar y clasificar las células del epitelio vaginal; en el experimento tres se aplicaron dos protocolos, Ovsynch y Ovsynch + Progesterona (O+P4); se realizó el mismo análisis del experimento 2 y se usó ecografía para diagnóstico de gestación. Para los resultados obtenidos se realizó estadística descriptiva y análisis de varianza ANOVA y prueba de Chi cuadrado (X2). En el Experimento uno: la edad influyó en las medidas absolutas de ovarios, cuernos uterinos, cérvix y conducto vaginal siendo mayores (p≤0,05) en cobayas de 12 semanas; las medidas relativas fueron mayores (p≤0,05) en la mayoría de estructuras en cobayas de 8 semanas. Experimento dos: existió dependencia (p<0,001) de la membrana vaginal con el tipo de células observadas. Experimento tres: ambos protocolos presentaron mayor porcentaje de células parabasales (47,85±21,22 % y 41,23±24,22 %); existió dependencia (p<0,001) entre la membrana vaginal, tipo de células y aplicación de protocolos. El porcentaje de gestación no mostró diferencia (p>0,05) entre tratamientos. Se concluye que la edad y los pesos de cobayas influyeron en las medidas absolutas y relativas de los órganos reproductores; la membrana vaginal está relacionada al tipo de células epitelio vaginales y los protocolos de sincronización, que, a pesar de no ser eficientes para presentar el estro, influyeron en la presentación del tipo de células y la membrana vaginal. Palabras claves: Ovsynch, cobaya, estro, útero, membrana vaginal.LojaSerrano Recalde, Elena Carolina2023-04-11T15:35:12Z2023-04-11T15:35:12Z2023-04-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis66 p.application/pdfhttps://dspace.unl.edu.ec/jspui/handle/123456789/26745spahttp://creativecommons.org/licenses/by-nc-sa/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Nacional de Lojainstname:Universidad Nacional de Lojainstacron:UNL2025-05-02T14:08:01Zoai:dspace.unl.edu.ec:123456789/26745Institucionalhttps://dspace.unl.edu.ec/Universidad públicahttps://unl.edu.ec/https://dspace.unl.edu.ec/oaiEcuador***opendoar:02025-05-02T14:08:01falseInstitucionalhttps://dspace.unl.edu.ec/Universidad públicahttps://unl.edu.ec/https://dspace.unl.edu.ec/oai*Ecuador***opendoar:02025-05-02T14:08:01Repositorio Universidad Nacional de Loja - Universidad Nacional de Lojafalse
spellingShingle Evaluación de dos protocolos de sincronización de celo en cuyes (Cavia porcellus)
Domínguez Pinta, Luis Fernando
OVSYNCH
COBAYA
MEMBRANA VAGINAL
status_str publishedVersion
title Evaluación de dos protocolos de sincronización de celo en cuyes (Cavia porcellus)
title_full Evaluación de dos protocolos de sincronización de celo en cuyes (Cavia porcellus)
title_fullStr Evaluación de dos protocolos de sincronización de celo en cuyes (Cavia porcellus)
title_full_unstemmed Evaluación de dos protocolos de sincronización de celo en cuyes (Cavia porcellus)
title_short Evaluación de dos protocolos de sincronización de celo en cuyes (Cavia porcellus)
title_sort Evaluación de dos protocolos de sincronización de celo en cuyes (Cavia porcellus)
topic OVSYNCH
COBAYA
MEMBRANA VAGINAL
url https://dspace.unl.edu.ec/jspui/handle/123456789/26745