Identificación de mycobacterium bovis mediante análisis microbiológico y molecular, en bovinos faenados en la ciudad de Loja

Bovine tuberculosis is a zoonotic infectious disease caused by Mycobacterium bovis that is characterized by producing granulomatous lesions in different organs, mainly lungs and local lymph nodes. In Ecuador, the demographic explosion has led the dairy industries to intensify production, which has favored the maintenance and spread of the infection. The aim of the present work was to identify Mycobac- terium bovis by microbiological and molecular analysis in cattle slaughtered in the city of Loja. 385 cattle slaughtered were considered for the study, of which during the post-mortem inspection, samples of the lungs, left tracheobronchial lymph no- des, and caudal mediastinal lymph nodes were taken, with no granulomatous lesions being recorded. The results obtained from the samples sown in the Stonebrink culture medium indicated the growth of 18 colonies with morphology compatible with Mycobacterium spp; 12 of which were identified as M. bovis (3.12 %) by multiplex PCR; the rest of the growths corresponded to 6 strains of environmental and atypical mycobacteria, which was determined by PCR-RFLP. The factors associated with the infection are determined considering a logistic regression analysis, considering the variables: race, age, sex, origin and lesions in lymph nodes and lung. Lymph node lesions were statistically associated with infection, having determined that the risk of identifying M bovis increases in nodules with hemorrhagic lesions compared to those with lesions such as anthracosis. Key words: bovine tuberculosis; Microbiological analysis; PCR; Loja

• 主要作者: Villavicencio Villavicencio, Daniela Cristina (author)
• 格式: bachelorThesis
• 語言: spa
• 出版: 2021
• 主題: BOVINA; TUBERCULOSIS; ANÁLISIS MICROBIOLÓGICO
• 在線閱讀: https://dspace.unl.edu.ec/jspui/handle/123456789/23792