Balance hormonal para la fase de brotación y enraizamiento in vitro de explantes de Cinchona officinalis L., proveniente de relictos boscosos de la provincia de Loja.
Cinchona officinalis L., known as keno or husk, it is endemic of Loja's valley, in the south of Ecuador, is one of the most important genres due to contain high quantity of alkaloids in its bark, which for centuries helped to fight malaria and malaria. Since then an excessive demand arose causi...
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| Format: | bachelorThesis |
| Jezik: | spa |
| Izdano: |
2018
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| Online dostop: | http://dspace.unl.edu.ec/jspui/handle/123456789/21599 |
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Označite | Izvleček: | Cinchona officinalis L., known as keno or husk, it is endemic of Loja's valley, in the south of Ecuador, is one of the most important genres due to contain high quantity of alkaloids in its bark, which for centuries helped to fight malaria and malaria. Since then an excessive demand arose causing the irrational exploitation of the species and destruction of its habitat, reason for which it is in danger of extinction and small relict forest. An alternative to contribute to the recovery and conservation of the species is to investigate new methods of in vitro propagation of plant tissues that allows multiply seedlings more quickly and efficiently. This research work was developed within the framework of the project: “Biotechnological processes to initiate genetic improvement of Cinchona officinalis L., from the relicts wooded province of Loja” and it was carried out in the laboratory of plant Micropropagation of the National University of Loja, where the correct hormonal balance was determined in the phases of the multiplication and rooting of Cinchona officinalis L. The vegetable material was taken from three wooded relicts of the province of Loja: Zamora Huayco (canton Loja), Uritusinga (canton Catamayo) and Selva Alegre (canton Saraguro). For the in vitro multiplication phase was used on the stem apexes and nodal segments from in vitro plants of Cinchona officinalis L., they were sown in the basal medium Murashige y Skoog (MS) supplemented with auxins and cytokinins in different concentrations: AIA (0,0; 0,2 and 0,5 mg L-1) and BAP (2,0 and 2,5 mg L-1). With this investigation one determined that the treatment T2 (0,0 mg L-1 AIA + 2,5 mg L-1 BAP) was the best as for percentage of brotation in three sectors Zamora Huayco with 97,78 %, Uritusinga 93,33 % and Selva Alegre reaching 78,89 %. In the in vitro rooting phase in the same way was used on the stem apexes and nodal segments of in vitro plants obtained in the phase of multiplication, where it was found that the concentration of the T2 (2,0 mg L-1 AIB + 0,0 mg L-1 BAP) was the greatest effect, obtaining in the sectors Zamora Huayco with 46,11%, Uritusinga 17,78 and Selva Alegre with 26,67% likewise, obtained that the T3 (1,0 mg L-1 AIB + 0,5 mg L-1 BAP) said the same percentage in this sector. In this way, it was determined that the use of higher concentration of cytokinins promotes the formation of shoots while, when combining high concentrations of auxins stimulate the formation of roots. Key words: Cinchona, cascarilla, micropropagation, multiplication, rooting. |
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