Determinación de la presencia del virus de newcastle en gallinas criollas del cantón Zapotillo, provincia de Loja
150 cloacal swabs distributed in 20 pools with 7 samples collected from each, these samples were from birds showing clinical signs of the disease, but more samples were obtained from healthy birds, which were utilized for performing viral isolation embryonated eggs from 9 to11 days, an alantoideo fl...
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| Váldodahkki: | |
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| Materiálatiipa: | bachelorThesis |
| Giella: | spa |
| Almmustuhtton: |
2014
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| Fáttát: | |
| Liŋkkat: | http://dspace.unl.edu.ec/jspui/handle/123456789/12362 |
| Fáddágilkorat: |
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Lasit fáddágilkoriid | Čoahkkáigeassu: | 150 cloacal swabs distributed in 20 pools with 7 samples collected from each, these samples were from birds showing clinical signs of the disease, but more samples were obtained from healthy birds, which were utilized for performing viral isolation embryonated eggs from 9 to11 days, an alantoideo fluid was used to analyze the performance of virus isolation in embryonated eggs via the hemagglutination test and confirmed by RT-PCR the addition of which the presence of the virus, can identify their pathogenicity , using specific primers which recognize the cut section of the protein F. Se those samples considered positive amplification presenting lengths 362 (bp) in which the existence of the Newcastle disease virus was found in 31,5 %, in the parishes of Cazaderos, Grey Heron, Mangahurco, Shoulders and Bolaspamba. Those samples that showed amplifications of 254 (bp) were considered to determine the pathogenicity of the virus, which resulted in strains that exist patógenas en Cazaderos parishes, Grey Heron, Shoulders and Bolaspamba. For detection of antibodies to a kit of ELISA frente Virus Newcastle disease in chicken serum from IDEXX Laboratories, test designed to measure the relative concentration of antibody to the virus NDV chicken serum. The serum samples obtained advanced degrees to 396 were considered positive. |
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