Evaluación de pre y post congelación de semen de un toro mestizo jersey utilizando optixcell más glicerol al 6 % y 8%
Freezing bovine semen is a key procedure to preserve the genetics of bulls, and its success depends on factors such as cryoprotective diluents. In this research, the effect of Optixcell combined with 6% and 8% glycerol on the semen quality of a Jersey crossbred bull before and after freezing was ana...
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| Formatua: | masterThesis |
| Hizkuntza: | spa |
| Argitaratua: |
2025
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| Gaiak: | |
| Sarrera elektronikoa: | https://dspace.unl.edu.ec/jspui/handle/123456789/32451 |
| Etiketak: |
Etiketa erantsi
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Etiketa erantsi | Gaia: | Freezing bovine semen is a key procedure to preserve the genetics of bulls, and its success depends on factors such as cryoprotective diluents. In this research, the effect of Optixcell combined with 6% and 8% glycerol on the semen quality of a Jersey crossbred bull before and after freezing was analyzed.The objective was to determine if these concentrations (0%, 6% and 8% glycerol) helped to improve parameters such as motility, vigor, viability and vitality. The study was carried out in Loja, Ecuador, with samples processed in the Animal Reproduction Laboratory of the National University of Loja. Three ml of semen were collected from a Jersey crossbred bull, obtaining 60 straws, distributed in three treatments. Samples were evaluated in the Androscope MINITUBE CASA program for sperm motility, vigor and viability. The process included collection by electroejaculation, dilution with Optixcell, progressive cooling, freezing with liquid nitrogen and rapid thawing in a water bath. An analysis of variance (ANOVA) was applied to compare the effects of glycerol. The results showed that, although there were no statistically significant differences between treatments, it was observed that sperm motility decreased in pre-freezing with the addition of glycerol, but improved after thawing, especially with 6%. Suggesting that, although glycerol may negatively affect initial motility, its cryoprotective action is manifested post-freezing. However, these observed improvements were not sufficient to conclude that glycerol has a decisive impact on semen quality. Nor were relevant differences in terms of viability and vitality found between glycerol concentrations. In conclusion, although 6% and 8% glycerol, together with Optixcell, favored the recovery of post-thawing motility, it did not generate significant improvements in seminal quality. |
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