Procesos biotecnológicos para la propagación in vitro de Cinchona officinalis L., a partir de diferentes fuentes de materia vegetal
The Cascarilla Cinchona officinalis L., is a native and endemic species of the Valley of Loja, it is considered as one of the most important genres for its medicinal value; which has caused over exploitation exceeding the limits of natural regeneration; The refore, it is in danger of extinction. Pla...
Αποθηκεύτηκε σε:
| Κύριος συγγραφέας: | |
|---|---|
| Μορφή: | bachelorThesis |
| Γλώσσα: | spa |
| Έκδοση: |
2016
|
| Θέματα: | |
| Διαθέσιμο Online: | http://dspace.unl.edu.ec/jspui/handle/123456789/13520 |
| Ετικέτες: |
Προσθήκη ετικέτας
Δεν υπάρχουν, Καταχωρήστε ετικέτα πρώτοι!
|
Προσθήκη ετικέτας | Περίληψη: | The Cascarilla Cinchona officinalis L., is a native and endemic species of the Valley of Loja, it is considered as one of the most important genres for its medicinal value; which has caused over exploitation exceeding the limits of natural regeneration; The refore, it is in danger of extinction. Plant micropropagation is a useful tool for the conservation of endangered species, by using the techniques of in vitro culture you can increase the number of individuals per unit area of any species. In this context the micropropagation of Cinchona officinalis L., was carried out in order to generate information on biotechnological processes, which allow the in vitro propagation of the species for conservation purposes. This research was developed within the framework of the project "identification and description of the current state of Cinchona officinalis L., in the province of Loja, and generation of protocols for in vivo and in vitro propagation", in two phases: field and laboratory; within the period between May 2015 - January 2016. The field phase corresponded to the collection of plant material in the areas of Loja: in the streams of the Naque and San Simon, Catamayo, Uritusinga sites and Saraguro in Selva Alegre parish, and the second phase was conducted in the laboratory of plant micropropagation of the Universidad Nacional de Loja. The laboratory phase was used in MS (Murashige and Skoog, 1962) basal culture medium, supplemented with different plant growth regulators. For disinfection of seed testing was applied (Ajax) sodium hypochlorite at three concentrations (15, 25 and 50%) in three times of immersion (5, 10 and 15 min.) respectively, where the best treatments to control pollution was 50% sodium hypochlorite for 5, 10 and 15 min. For the in vitro germination of seeds was added to the culture medium (AG3) gibberellic acid in three concentrations (0,0; 0,5 and 1,0 mg/l); finding the highest percentage of germination (74,44%) in T3, with a concentration of 1,0 mg/l of AG3. To test in vitro propagation of explants, used three regulators of growth, the Auxin: naphthaleneacetic acid (ANA) and two cytokinins: benzilaminopurina (BAP) and kinetin (KIN), in two concentrations 0,2 and 2,0 mg/l respectively, achieving the best results in the hormone combination of 0,2 ANA 2.0 BAP; the same one that allowed 4,73 developed buds, with 0,83 cm height, 27 leaves and 12,10 average knots formed by each explant. This research allowed to rehearse the biotechnological processes for the in vitro propagation of Cinchona officinalis L. in order to undertake in the future programs of breeding, afforestation and reforestation of the species, which allow the recovery of native ecosystems degraded especially in the Southern region of the Ecuador. |
|---|