Protocolo para la propagación in vitro de arándano (Vaccinium corymbosum L.) cv. Biloxi

The blueberry (Vaccinium corymbosum L.) is a fruit of great importance due to its high antioxidant content, which makes it the fourth most economically important fruit worldwide. The growth in demand for blueberries in recent years has created opportunities to diversify production in the country. However, difficulties have been identified for its sexual reproduction and rooting of cuttings, which makes in vitro growing an effective tool to counteract the reproductive limitations of this species. In this context, the following research aimed to develop a protocol for in vitro propagation of blueberry from field explants. During the in vitro establishment stage, explants were subjected to a pre-disinfection; they were immersed in fungicide (Azoxystrobin + frutiafol 3 ml.L-1) for 3 hours. Six disinfection treatments were tested using sodium hypochlorite as disinfectant agent at different concentrations (10%, 15% and 20%) during two immersion times (10 and 20 minutes). After establishing the disinfection protocol, the effect of two culture media: WPM and MS together with two cytokinins: kinetin and 2ip at a concentration of 2 mg L-1 on shoot development was evaluated and compared. In both cases, a completely randomized design was used. The results obtained showed that the disinfection treatment with 15 % sodium hypochlorite for 10 minutes proved to be the most effective in the disinfection of blueberry explants, with only 20 % loss between contaminated and phenolized explants. The control treatment with WPM basal medium without growth regulator had the highest values for shoot length and number of leaves at 60 days.

• Autor principal: Cueva Ramos, Leidy Stefania (author)
• Formato: bachelorThesis
• Lenguaje: spa
• Publicado: 2024
• Materias: PROPAGACION IN VITRO; BROTACION; CITOQUININAS
• Acceso en línea: https://dspace.unl.edu.ec/jspui/handle/123456789/28750