Actividad leishmanicida del aceite esencial de Plumbago scandens L. en promastigotes de Leishmania major like
Cutaneous and mucocutaneous Leishmaniasis is a disease caused by several species of the genus Leishmania and transmitted by the bite of various hematophagous species of the genus Lutzomia. For its treatment, pentavalent antimonial drugs such as N-methylglucamine (Glucantime) are used, which are the...
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| Format: | bachelorThesis |
| Idioma: | spa |
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2018
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| Accés en línia: | http://dspace.unl.edu.ec/jspui/handle/123456789/21017 |
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Afegir etiqueta | _version_ | 1857833074400690176 |
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| author | Puchaicela Quirola, Jorge David |
| author_facet | Puchaicela Quirola, Jorge David |
| author_role | author |
| collection | Repositorio Universidad Nacional de Loja |
| dc.contributor.none.fl_str_mv | Morocho Yaguana, Luis Alberto |
| dc.creator.none.fl_str_mv | Puchaicela Quirola, Jorge David |
| dc.date.none.fl_str_mv | 2018-08-24T13:27:12Z 2018-08-24T13:27:12Z 2018 |
| dc.format.none.fl_str_mv | 73 p. application/pdf |
| dc.identifier.none.fl_str_mv | http://dspace.unl.edu.ec/jspui/handle/123456789/21017 |
| dc.language.none.fl_str_mv | spa |
| dc.rights.none.fl_str_mv | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Nacional de Loja instname:Universidad Nacional de Loja instacron:UNL |
| dc.subject.none.fl_str_mv | LEISHMANIASIS FACTORES - RIESGO LEISHMANIASIS |
| dc.title.none.fl_str_mv | Actividad leishmanicida del aceite esencial de Plumbago scandens L. en promastigotes de Leishmania major like |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | Cutaneous and mucocutaneous Leishmaniasis is a disease caused by several species of the genus Leishmania and transmitted by the bite of various hematophagous species of the genus Lutzomia. For its treatment, pentavalent antimonial drugs such as N-methylglucamine (Glucantime) are used, which are the first choice. Alternatively, in traditional medicine empirical medicinal plants are used to treat this disease, without an entire knowledge about its benefits and mechanism of action, being one of them Plumbago scandens L. (Plumbaguinaceae). The present study was designed to evaluate the leishmanicidal activity of the essential oil of Plumbago scandens L. which was extracted from the aerial parts (stems and leaves) by steam distillation and was evaluated its activity on axenic promastigotes of L. major like and the percentage of growth inhibition of the parasites determinated. The determination of the inhibition percentage was based on the metabolic reduction of 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazole bromide (MTT) by mitochondrial succinate-dehydrogenase to a blue-violet colored compound, with maximum absorbance at 570 nm. The reference drug used was amphotericin B. The results showed that the essential oil inhibits in 85% the development of the parasite at concentrations of 15-30 μg/ml, causing the lysis of these and inhibiting their reproduction and motility, at the same time, amphotericin alone caused the inhibition of its division; the results obtained in the research were socialized to Students and Teachers of the Clinical Laboratory Career of National University of Loja. Keywords: Leishmania major like, leishmanicide, Leishmania, essential oil, Plumbago scandens L, MTT |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UNL_ba8a3cac4e1a34db06715d4881b16af0 |
| instacron_str | UNL |
| institution | UNL |
| instname_str | Universidad Nacional de Loja |
| language | spa |
| network_acronym_str | UNL |
| network_name_str | Repositorio Universidad Nacional de Loja |
| oai_identifier_str | oai:dspace.unl.edu.ec:123456789/21017 |
| publishDate | 2018 |
| reponame_str | Repositorio Universidad Nacional de Loja |
| repository.mail.fl_str_mv | * |
| repository.name.fl_str_mv | Repositorio Universidad Nacional de Loja - Universidad Nacional de Loja |
| repository_id_str | 0 |
| rights_invalid_str_mv | http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ |
| spelling | Actividad leishmanicida del aceite esencial de Plumbago scandens L. en promastigotes de Leishmania major likePuchaicela Quirola, Jorge DavidLEISHMANIASISFACTORES - RIESGO LEISHMANIASISCutaneous and mucocutaneous Leishmaniasis is a disease caused by several species of the genus Leishmania and transmitted by the bite of various hematophagous species of the genus Lutzomia. For its treatment, pentavalent antimonial drugs such as N-methylglucamine (Glucantime) are used, which are the first choice. Alternatively, in traditional medicine empirical medicinal plants are used to treat this disease, without an entire knowledge about its benefits and mechanism of action, being one of them Plumbago scandens L. (Plumbaguinaceae). The present study was designed to evaluate the leishmanicidal activity of the essential oil of Plumbago scandens L. which was extracted from the aerial parts (stems and leaves) by steam distillation and was evaluated its activity on axenic promastigotes of L. major like and the percentage of growth inhibition of the parasites determinated. The determination of the inhibition percentage was based on the metabolic reduction of 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazole bromide (MTT) by mitochondrial succinate-dehydrogenase to a blue-violet colored compound, with maximum absorbance at 570 nm. The reference drug used was amphotericin B. The results showed that the essential oil inhibits in 85% the development of the parasite at concentrations of 15-30 μg/ml, causing the lysis of these and inhibiting their reproduction and motility, at the same time, amphotericin alone caused the inhibition of its division; the results obtained in the research were socialized to Students and Teachers of the Clinical Laboratory Career of National University of Loja. Keywords: Leishmania major like, leishmanicide, Leishmania, essential oil, Plumbago scandens L, MTTLa Leishmaniasis cutánea y mucocutánea es una enfermedad producida por varias especies del género Leishmania y trasmitida por la picadura de diversas especies hematófagos del genero Lutzomia. Para su tratamiento se utilizan fármacos antimoniales pentavalentes como la N-metilglucamina (Glucantime) que son de primera elección. Alternativamente, en la medicina tradicional se usan empíricamente plantas medicinales para tratar esta enfermedad, sin un entero conocimiento sobre sus beneficios y mecanismo de acción, siendo una de ellas Plumbago scandens L(Plumbaguinaceae). El presente estudio se planteó evaluar la actividad leishmanicida del aceite esencial de la planta Plumbago scandens L. el cual fue extraído de las partes aéreas (tallos y hojas), por medio de la técnica de arrastre de vapor, y se evaluó sobre promastigotes axénicos de L. major like, mediante la determinación del porcentaje de inhibición del crecimiento de los parásitos. La determinación del porcentaje de inhibición se basó en la reducción metabólica del bromuro de 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol (MTT) por acción de la succinato-deshidrogenasa mitocondrial a un compuesto coloreado de color azul-violeta con absorbancia máxima a 570 nm. El fármaco de referencia usado fue la anfotericina B. Los resultados mostraron que el aceite esencial inhibe en un 85% el desarrollo del parásito a concentraciones de 15-30 µg/ml, provocando la lisis de éstos e inhibiendo su reproducción y motilidad, al tiempo que la anfotericina solo causó la inhibición de su división; los resultados obtenidos en la investigación fueron socializados a estudiantes y Docentes de la Carrera de Laboratorio Clínico. Palabras clave: Leishmania major like, leishmanicida, Leishmania, aceite esencial, Plumbago scandens L, MTT.Morocho Yaguana, Luis Alberto2018-08-24T13:27:12Z2018-08-24T13:27:12Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis73 p.application/pdfhttp://dspace.unl.edu.ec/jspui/handle/123456789/21017spahttp://creativecommons.org/licenses/by-nc-sa/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Nacional de Lojainstname:Universidad Nacional de Lojainstacron:UNL2025-05-02T16:00:13Zoai:dspace.unl.edu.ec:123456789/21017Institucionalhttps://dspace.unl.edu.ec/Universidad públicahttps://unl.edu.ec/https://dspace.unl.edu.ec/oaiEcuador***opendoar:02025-05-02T16:00:13falseInstitucionalhttps://dspace.unl.edu.ec/Universidad públicahttps://unl.edu.ec/https://dspace.unl.edu.ec/oai*Ecuador***opendoar:02025-05-02T16:00:13Repositorio Universidad Nacional de Loja - Universidad Nacional de Lojafalse |
| spellingShingle | Actividad leishmanicida del aceite esencial de Plumbago scandens L. en promastigotes de Leishmania major like Puchaicela Quirola, Jorge David LEISHMANIASIS FACTORES - RIESGO LEISHMANIASIS |
| status_str | publishedVersion |
| title | Actividad leishmanicida del aceite esencial de Plumbago scandens L. en promastigotes de Leishmania major like |
| title_full | Actividad leishmanicida del aceite esencial de Plumbago scandens L. en promastigotes de Leishmania major like |
| title_fullStr | Actividad leishmanicida del aceite esencial de Plumbago scandens L. en promastigotes de Leishmania major like |
| title_full_unstemmed | Actividad leishmanicida del aceite esencial de Plumbago scandens L. en promastigotes de Leishmania major like |
| title_short | Actividad leishmanicida del aceite esencial de Plumbago scandens L. en promastigotes de Leishmania major like |
| title_sort | Actividad leishmanicida del aceite esencial de Plumbago scandens L. en promastigotes de Leishmania major like |
| topic | LEISHMANIASIS FACTORES - RIESGO LEISHMANIASIS |
| url | http://dspace.unl.edu.ec/jspui/handle/123456789/21017 |