Identificación de los microorganismos asociados a la rizósfera del babaco (Vasconcella heilbornii. var. pentágona) en la provincia de Loja

Agricultural activity and food security stand out as main activities within the national economy and have become increasingly important in recent years within Ecuador. There is a boom in the production of non-traditional and ancestral crops and one of these is Babaco from the valleys of the province of Loja. This research aimed to identify the microorganisms in the rhizosphere of Babaco (Vasconcellea heilbornii var. pentagona) in the province of Loja, using traditional methods. For this purpose, soil samples were taken from Babaco plantations in Chuquiribamba and Saraguro to obtain isolates in order to conduct morphological characterization and ascertain their biological effectiveness as an antagonist against Fusarium oxysporum. Isolates were extracted from a total of nine soil samples using serial dilution. Subsequently, from the purified isolates the morphometric identification of each fungal potential antagonist MVB was conducted. Based on their morphological and macroscopic characteristics, taking into account their appearance, color and surface of the colony grown on a PDA medium, bacteria testing was performed for characterization identification in Kliger, MacConkey, catalase, gram staining agars, LPGA agar and slant agar. Eventually quantitative tests of fungal antagonism using two isolates of Fusarium oxysporum (ALM2 and QUI2) were performed against Penicillium sp for its high content of conidia and for the Fungal Bacterium confrontation, the same isolates of Fusarium oxysporum against the Bacillus sp isolates were used to demonstrate the best macro, microscopic and biochemical characteristics. The relevance of this work is part of creating guidelines for the determination of rhizosphere microorganisms’ antagonists causing soil fungi diseases in economically important crops such as Babaco.

• Huvudupphovsman: Chiriapo Guarderas, Claudia Irma (author)
• Materialtyp: bachelorThesis
• Språk: spa
• Publicerad: 2016
• Ämnen: BABACO; MICROORGANISMOS ANTAGONISTAS; MARCHITEZ VASCULAR; FUSARIUM OXYSPORUM
• Länkar: http://dspace.unl.edu.ec/jspui/handle/123456789/14979