Efecto del fotoperíodo en la inducción de estructuras callogénicas en explantes de coffea arabica l., a partir de plántulas de invernadero, a nivel de laboratorio

2.1 ABSTRACT Coffea arabica L. is a species native to East Africa, cultivated worldwide. In Ecuador it is important economically, socially and environmentally, but faces challenges such as low productivity and ageing crops. The present research was developed with the purpose of generating information for the in vitro propagation of coffee, in the formation of callogenic structures from leaf explants, evaluating the effect of photoperiod and the hormones 2,4-D (2,4-dichlorophenoxyacetic acid) and KIN (Kinetin). In the first trial, leaf explants of one square centimetre, were obtained from coffee seedlings grown in greenhouses, which were treated with Benomil and Kasumin. Subsequently, the explants were sown in MS culture medium (Murashige and Skoog, 1962), supplemented with vitamin B5 complex (thiamine, myo-inositol, pyridoxine, nicotinic acid, glycine), Ergostim, sucrose and agar; and, with different concentrations of 2,4-D (auxin) and Kinetin (cytokinin), according to the 8 treatments. In the second trial, the in vitro corns were sown in the best culture medium previously obtained, but with the hormones reduced by half (2,4-D and KIN), half of the trial was subjected to photoperiod (16 hours of light) and the rest to total darkness, according to the 4 treatments. Each trial had a completely randomised bifactorial design and 3 replications. Finally, the combination 1.0 mg L-1 2,4-D + 0.5 mg L-1 KIN reached the highest callus formation, with 15.56 %; while the combination 0.5 mg L-1 2,4-D + 0.25 mg L-1 KIN in total darkness obtained the highest proliferation, with 43.3 %, proving the capacity of C. arabica L. to generate callus tissue in the presence of phytoregulators. Key words: Callogenesis, Rubiaceae, daylight hours, phytoregulator, induction, proliferation.

• Үндсэн зохиолч: Cueva Jiménez, Olger Vinicio (author)
• Формат: bachelorThesis
• Хэл сонгох: spa
• Хэвлэсэн: 2022
• Нөхцлүүд: COFFEA ARABICA; INDUCCION
• Онлайн хандалт: https://dspace.unl.edu.ec/jspui/handle/123456789/25009