Evaluación de soportes orgánicos en interacción con bacterias promotoras del crecimiento vegetal para la formulación de un prototipo de inoculante

Conventional agricultural management has caused irreversible damage to soil quality and environmental health, forcing the implementation of a new model of sustainable environmental practices. Biofertilisers have been identified as a biotechnological alternative to increase soil fertility and crop production in sustainable agriculture. Beneficial microorganisms need a support that provides them with protection and viability from the moment of biofertiliser production to its application in the field. Through this research it was possible to evaluate the organic supports humus, peat and alginate beads as carriers of native microorganisms: Azospirillum spp., Azotobacter spp. and Pseudomonas spp. and the best support was selected for the formulation of an inoculant prototype. The results obtained in the behaviour of the supports in interaction with the plant growth promoting bacteria at initial concentrations were: Azotobacter spp. of 1.2x109, for Azospirillum spp. 7.7 x 107 and in Pseudomonas spp. 3.3x108 CFU g-1. The T4 control Nutrient Broth (NC) plus Azotobacter spp. from 35 days after inoculation (DDI) to 80 DDI showed the highest values. Followed by T2 humus showed the highest values at 50 DDI and 65 DDI. T4 control (CN) plus Azospirillum spp. showed the highest values from 35 DDI to 80 DDI. This was followed by T3 (alginate) inoculated with Azospirillum spp. where the highest values were observed from 20 DDI to 65 DDI. T3 Pseudomonas spp. with alginate beads showed the highest values during the 80 days. Likewise, T2 Pseudomonas spp. with humus showed the highest values from 35 DDI to 80 DDI. The perch viability for T4 and T2 Azotobacter spp., T3 and T4 Azospirillum spp. and T3 Pseudomonas spp. maintained their bacterial concentration up to 155 DDI, while T2 Pseudomonas spp. at 155 DDI lowered its value by one logarithmic unit. The highest encapsulation potential was achieved with the alginate beads for Azospirillum spp. and Pseudomonas spp. strains with a viability up to 155 DDI, while the humus support in interaction with Azotobacter spp. and Pseudomonas spp. had the highest survival up to 65 DDI and 110 DDI respectively. Keywords: organic media, inoculant, Azospirillum spp., Azotobacter spp. and Pseudomonas spp., nutrient broth.

• Autor principal: Guillén Guzmán, Franco Antonio (author)
• Format: masterThesis
• Idioma: spa
• Publicat: 2023
• Matèries: SOPORTE ORGANICO; BACTERIAS
• Accés en línia: https://dspace.unl.edu.ec/jspui/handle/123456789/27185