Caracterización e identificación de cepas nativas del género Azotobacter y su efecto en co-inoculación con Rhizobium en tomate de mesa
This research aimed to characterize and identify native strains of the genus Azotobacter and its effect on co-inoculation with Rhizobium in tomatoes in a greenhouse. For this purpose, locations within the Sosoranga and Cariamanga, cantons were sampled and geo-referenced using Global Positioning Syst...
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2015
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| Subjects: | |
| Acceso en liña: | http://dspace.unl.edu.ec/jspui/handle/123456789/12259 |
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Engadir etiqueta | Summary: | This research aimed to characterize and identify native strains of the genus Azotobacter and its effect on co-inoculation with Rhizobium in tomatoes in a greenhouse. For this purpose, locations within the Sosoranga and Cariamanga, cantons were sampled and geo-referenced using Global Positioning System (GPS). The establishment of bacterial isolates was conducted by means of morphological characterization of the isolated colonies and by using Gram staining, the growth, color, mucus production, and elevated edges were evaluated. The physiological characterization consisted of quantification of each isolate in the production of indole acetic acid (IAA) and solubilization of P. The genetic identification of isolates resulting from the morphological and physiological characterization was done by partial sequencing of the genes of the 16S rRNA sub region. From a total of 14 initial isolates 6 bacterial genera were identified, Stenotrophomonas, Sphingomonas, Azotobacter, Comamonas, Ralstonia and Rhizobium. All isolates were able to produce IAA and not solubilize phosphorus. The best results for the morphological parameters as to the height and number of leaves were obtained with the T3 inoculation (Rhizobium sp.). With respect to the variable number of flowers after 60 days after sowing (DAS) was T1 (Azotobacter vinelandii + Rhizobium sp.) and the variable number of fruit at 60 and 75 DAS increased with the inoculation of T2 (Azotobacter vinelandii). With respect to biomass parameters, these were influenced positively with the T3 (Rhizobium sp.) after 75 DDS, with the fresh weight of root (FWR) being 19.75 g and the fresh weight of the foliage (FWF) being 113.14 g. With respect to the fresh fruit weight (FFW) the best treatment corresponded to T2 (Azotobacter vinelandii) at 39.95 g. Treatment T3 (Rhizobium sp.) provided the most positive results with respect to the dry weight of the root (DWR) at 1.46 g and 14.70 g of dry weight of foliage (DWF). |
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