Enraizamiento hidropónico e in vivo de cinchona officinalis l., a partir de plántulas ex vitro
Ecuador, being a megadiverse country, is home to a great variety of endemic species, one of them is Cinchona officinalis L., which is distributed in the province of Loja and commonly known as the "Tree of Life" which is of great historical and cultural value for the province and the countr...
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| Formato: | bachelorThesis |
| Lenguaje: | spa |
| Publicado: |
2023
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| Materias: | |
| Acceso en línea: | https://dspace.unl.edu.ec/jspui/handle/123456789/27609 |
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Agregar Etiqueta | Sumario: | Ecuador, being a megadiverse country, is home to a great variety of endemic species, one of them is Cinchona officinalis L., which is distributed in the province of Loja and commonly known as the "Tree of Life" which is of great historical and cultural value for the province and the country. This species has medicinal properties that were used to cure malarial fevers in the 17th century; at present, several factors have contributed to the degradation of the species: low rate of natural regeneration, limitations for propagation by conventional methods, high mortality when establishing plantations, etc. For this reason, the present research was aimed at using alternative techniques for in vivo rooting of Cinchona officinalis L.; for this purpose, two trials were set up with different concentrations of chemically pure hormones and a commercial rooter. In the first trial, Cinchona micro-stakes were planted using the hydroponic method, with the addition of two hormones: indolacetic acid (IAA) and hormone agrimon 1 (HG 1); and, in the second trial, they were planted in peat, with the two hormones mentioned above. The following variables were evaluated: percentage of contamination, survival, root formation, number and average length of roots, and of shoots per micro-stake. The results obtained determined that hydroponic rooting in treatment T2 (3.0 g L-1 AIA + 0.0 g L-1 HG 1) was the most effective, presenting 93.3% rooting, with an average number of roots of 10.06 and an average length of 3.58 cm. In addition, the hormone concentration (1.5 g L-1 AIA + 0.0 g L-1 HG 1), belonging to the T1 treatment of the AIA and HG 1 hormone balance, obtained 1.5 shoots per micro-stake, with a length of 0.06 cm. Similarly, in the trial with peat, it was observed that T2, with 3.0 g L-1 AIA + DM, registered the highest percentage of root formation in the micro-stakes, with 46.67 %, an average number of roots of 6.80 and a length of 1.7 cm; in contrast to the control treatment (T0), where 1.27 shoots per micro-stake and a length of 0.20 cm were observed. In conclusion, auxin or chemically known as indoleacetic acid (AIA) induced the proliferation of adventitious roots and shoot formation, both in hydroponics and peat; likewise, it was present in the T0 treatment without the application of rooting hormones. Finally, based on the results obtained, it is recommended to test and determine the appropriate hormonal balance for root formation in micro-stakes of Cinchona officinalis L., both in the hydroponic method and in peat. Keywords: Cinchona officinalis L., rooting, micro-stakes, auxins, hydroponics, peat. |
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