Evaluación de la composición química y actividad biológica del aceite esencial proveniente de las hojas de Piper barbatum Kunth (cordoncillo).

This research evaluated the chemical composition and biological activity of essential oil from the leaves of Piper barbatum Kunth. Distillation was carried out with the clevenger hydrodistillation equipment for obtaining the oil, which was analyzed by gas chromatography coupled to masses to characterize the chemical components. The antioxidant activity was determined by the methods ABTS and DPPH using as a natural pattern the essential oil of thyme Thymus vulgaris. The results showed that Piper barbatum Kunth oil presented low free radical collecting capacity with (IC50 DPPH 51.47 ± 0.35 mg/mL; IC50 ABTS 127.83 ± 0.40 mg/ml) compared with the natural reference Thymus vulgaris with (IC50 DPPH 0.17 ± 0.005 mg/ml; IC50 ABTS 0.53 ± 0.24 mg/ML) which was upper. The antimicrobial activity was performed by the method of dilution or micro-dilution in broth, calculating the minimum inhibitory concentration (MIC) in 10 bacterial strains and 2 yeasts, as controls were used essential oils Thymus vulgaris, citrate and antibiotics: erythromycin, gentamicin and as antifungal clotrimoxasol. The best results were obtained in: Streptococcus mutans ATCC 25175 and Candida albicans ATCC 10231 with (MIC 132 μg/mL), followed by Candida tropicalis ATCC 13803, Staphylococcus aureus ATCC 29213 (MIC 264 μg/ml), Micrococcus luteus ATCC 4698, Staphylococcus epidermidis ATCC 14990, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027 with (MIC 528 μg/ml).

• Autor principal: Veloz Jaramillo, Jennifer Tatiana (author)
• Format: bachelorThesis
• Idioma: spa
• Publicat: 2018
• Matèries: BIOTECNOLOGÍA; ACEITES ESENCIALES; PIPER BARBATUM; BOTÁNICA
• Accés en línia: http://dspace.ups.edu.ec/handle/123456789/16382