Evaluación de la eficacia de Trichoderma sp y Pseudomona sp para biorremediación de suelos contaminados con hidrocarburos

The aim of this research was evaluate the power of bioremediation of the fungus Trichoderma sp and the bacteria Pseudomonas fluorescens in a sample of soil contaminated by hydrocarbons, in order to reduce the TPH (total polycyclic hydrocarbons) in soil. This project was completed in two phases: the field work consisted of soil sampling and the laboratory work consisted of installation of an assay with a DBCA system with 4 treatments (T0: control soil, T1: Soil + Trichoderma sp, T2: Soil + Pseudomonas fluorescens and T3 : Soil with Pseudomonas fluorescens + Trichoderma sp ) and 4 repetitions with controlled environmental conditions such as pH, temperature, electrical conductivity and moisture; the physico-chemical and TPH analyzes was performed at beginning and end of the assay for 3 months. The soil at beginning of the assay had macro and micro elements which are essential for microorganisms to degrade hydrocarbons. The initial TPH contamination in soil was 42070.3 mg / kg, and at the end of the assay the T1, T2 and T3 obtained an effective remediation, settling down within the permissible limits of RAOHE (environmental regulations hydrocarbon activities); demonstrating that all treatments work independently and in a consortium. The T3 (consortium) was the treatment that degraded more amount of hydrocarbons with a value of 379.2 mg / kg, the reason was because both microorganisms had the necessary mechanisms to degrade the hydrocarbon molecules, showing that bioremediation is an ecological and effective process for the degradation of hydrocarbons.

• Egile nagusia: Muñoz Cuaical, Stalin David (author)
• Formatua: bachelorThesis
• Hizkuntza: spa
• Argitaratua: 2016
• Gaiak: BIOTECNOLOGÍA; TRICHODERMA; PSEUDOMONA; SUELOS - ANÁLISIS
• Sarrera elektronikoa: https://dspace.ups.edu.ec/handle/123456789/13228