Actividad alexitera de Tabernaemontana sananho Ruiz & Pav. (Kunapi) y Minthostachys cf. mollis (kunth) Griseb (Kurarina) sobre el veneno de Bothrops atrox (“Pitalala”)
Ecuador has abundant and diverse flora and fauna in its four geographic regions. In the Ecuadorian Amazon inhabits the snake Bothrops atrox, that caused the highest number of accidents by bites registered; inhabitants of this region ensure that plants such as Kunapi (Tabernaemontana sananho Ruiz &am...
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| Формат: | bachelorThesis |
| Мова: | spa |
| Опубліковано: |
2017
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| Онлайн доступ: | http://dspace.ups.edu.ec/handle/123456789/14639 |
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Додати тег | _version_ | 1858988421175836672 |
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| author | León Cárdenas, Karen Priscila |
| author_facet | León Cárdenas, Karen Priscila |
| author_role | author |
| collection | Repositorio Universidad Politécnica Salesiana |
| dc.contributor.none.fl_str_mv | Tapia Hernández, Wilson Fabián |
| dc.creator.none.fl_str_mv | León Cárdenas, Karen Priscila |
| dc.date.none.fl_str_mv | 2017-09-14T20:09:34Z 2017-09-14T20:09:34Z 2017-09 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dspace.ups.edu.ec/handle/123456789/14639 |
| dc.language.none.fl_str_mv | spa |
| dc.rights.none.fl_str_mv | Atribución-NoComercial-SinDerivadas 3.0 Ecuador http://creativecommons.org/licenses/by-nc-nd/3.0/ec/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Politécnica Salesiana instname:Universidad Politécnica Salesiana instacron:UPS |
| dc.subject.none.fl_str_mv | BIOTECNOLOGÍA ACTIVIDAD ALEXÍTERA TABERNAEMONTANA SANANHO BOTHORPS ATROX |
| dc.title.none.fl_str_mv | Actividad alexitera de Tabernaemontana sananho Ruiz & Pav. (Kunapi) y Minthostachys cf. mollis (kunth) Griseb (Kurarina) sobre el veneno de Bothrops atrox (“Pitalala”) |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | Ecuador has abundant and diverse flora and fauna in its four geographic regions. In the Ecuadorian Amazon inhabits the snake Bothrops atrox, that caused the highest number of accidents by bites registered; inhabitants of this region ensure that plants such as Kunapi (Tabernaemontana sananho Ruiz & Pav) and Kurarina (Minthostachys mollis (kunth) Griseb) are commonly used to counteract said effect. In this investigation, it was determined in vitro the neutralizing activity of the extracts of these two-plant species on the effects of the venom of B. atrox. The Samples of these plants were collected in Huamboya and Morona, cantons of the province of Morona Santiago, from which, were prepared dry total extracts, alcoholic and aqueous to test the anti-coagulant activity and anti-proteolytic, using different concentrations of the extracts on a defined dose of the venom of B. atrox; in addition, the electrophoretic profile was determined, before and after the action of the extracts. The results obtained from anticoagulant activity revealed that a concentration of 0,25 ppm of the alcoholic extract of both species is sufficient to neutralize the coagulant effect of a dissolution 0.02 mg/ml, of poison by increasing the clotting time of the plasma by 500%. The results of anti-proteolytic activity evidenced that the aqueous extract of T. Sananho was more effective and that M. mollis does not have this activity. The electrophoretic profile determined that the alcoholic extracts of the two-species changed the protein profile by the appearance of a band for a protein of 45,8 kDa. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UPS_cd9e008cc4935ff22df447437e623136 |
| instacron_str | UPS |
| institution | UPS |
| instname_str | Universidad Politécnica Salesiana |
| language | spa |
| network_acronym_str | UPS |
| network_name_str | Repositorio Universidad Politécnica Salesiana |
| oai_identifier_str | oai:dspace.ups.edu.ec:123456789/14639 |
| publishDate | 2017 |
| reponame_str | Repositorio Universidad Politécnica Salesiana |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Politécnica Salesiana - Universidad Politécnica Salesiana |
| repository_id_str | 1737 |
| rights_invalid_str_mv | Atribución-NoComercial-SinDerivadas 3.0 Ecuador http://creativecommons.org/licenses/by-nc-nd/3.0/ec/ |
| spelling | Actividad alexitera de Tabernaemontana sananho Ruiz & Pav. (Kunapi) y Minthostachys cf. mollis (kunth) Griseb (Kurarina) sobre el veneno de Bothrops atrox (“Pitalala”)León Cárdenas, Karen PriscilaBIOTECNOLOGÍAACTIVIDAD ALEXÍTERATABERNAEMONTANA SANANHOBOTHORPS ATROXEcuador has abundant and diverse flora and fauna in its four geographic regions. In the Ecuadorian Amazon inhabits the snake Bothrops atrox, that caused the highest number of accidents by bites registered; inhabitants of this region ensure that plants such as Kunapi (Tabernaemontana sananho Ruiz & Pav) and Kurarina (Minthostachys mollis (kunth) Griseb) are commonly used to counteract said effect. In this investigation, it was determined in vitro the neutralizing activity of the extracts of these two-plant species on the effects of the venom of B. atrox. The Samples of these plants were collected in Huamboya and Morona, cantons of the province of Morona Santiago, from which, were prepared dry total extracts, alcoholic and aqueous to test the anti-coagulant activity and anti-proteolytic, using different concentrations of the extracts on a defined dose of the venom of B. atrox; in addition, the electrophoretic profile was determined, before and after the action of the extracts. The results obtained from anticoagulant activity revealed that a concentration of 0,25 ppm of the alcoholic extract of both species is sufficient to neutralize the coagulant effect of a dissolution 0.02 mg/ml, of poison by increasing the clotting time of the plasma by 500%. The results of anti-proteolytic activity evidenced that the aqueous extract of T. Sananho was more effective and that M. mollis does not have this activity. The electrophoretic profile determined that the alcoholic extracts of the two-species changed the protein profile by the appearance of a band for a protein of 45,8 kDa.Ecuador cuenta con una flora y fauna mega diversa en sus cuatro regiones geográficas. En la amazonia ecuatoriana habita la serpiente Bothrops atrox, causante del mayor número de accidentes por mordeduras registradas; habitantes de ésta región aseguran que plantas como Kunapi (Tabernaemontana sananho Ruiz & Pav) y Kurarina (Minthostachys cf. mollis (kunth) Griseb) son de uso común para contrarrestar mencionado efecto. En ésta investigación se determinó in vitro la actividad neutralizante de los extractos de estas especies vegetales sobre los efectos del veneno de B. atrox. Las muestras fueron colectadas en los cantones Morona y Huamboya de la provincia de Morona Santiago, a partir de las cuales, se elaboraron extractos totales secos, alcohólicos y acuosos para ensayar actividad anti coagulante y anti proteolítica, utilizando diferentes concentraciones de los extractos sobre una dosis definida del veneno; además se determinó el perfil electroforético, antes y después de la acción de los extractos. Los resultados obtenidos de actividad anti coagulante revelaron que una concentración de 0,25 ppm del extracto alcohólico de ambas especies vegetales es suficiente para neutralizar el efecto coagulante de una disolución de 0,02 mg/mL, de veneno aumentando el tiempo de coagulación del plasma en un 500%. Los resultados de actividad anti proteolítica evidenciaron que el extracto acuoso de T. sananho fue más eficaz y que M. mollis no tiene ésta actividad. El perfil electroforético determinó que los extractos alcohólicos de las dos especies cambiaron el perfil proteico por aparición de una banda para una proteína de 45,8 kDa.Tapia Hernández, Wilson Fabián2017-09-14T20:09:34Z2017-09-14T20:09:34Z2017-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttp://dspace.ups.edu.ec/handle/123456789/14639spaAtribución-NoComercial-SinDerivadas 3.0 Ecuadorhttp://creativecommons.org/licenses/by-nc-nd/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Politécnica Salesianainstname:Universidad Politécnica Salesianainstacron:UPS2019-01-01T19:13:03Zoai:dspace.ups.edu.ec:123456789/14639Institucionalhttps://dspace.ups.edu.ec/Institución privadahttps://www.ups.edu.ec/https://dspace.ups.edu.ec/oai.Ecuador...opendoar:17372019-01-01T19:13:03Repositorio Universidad Politécnica Salesiana - Universidad Politécnica Salesianafalse |
| spellingShingle | Actividad alexitera de Tabernaemontana sananho Ruiz & Pav. (Kunapi) y Minthostachys cf. mollis (kunth) Griseb (Kurarina) sobre el veneno de Bothrops atrox (“Pitalala”) León Cárdenas, Karen Priscila BIOTECNOLOGÍA ACTIVIDAD ALEXÍTERA TABERNAEMONTANA SANANHO BOTHORPS ATROX |
| status_str | publishedVersion |
| title | Actividad alexitera de Tabernaemontana sananho Ruiz & Pav. (Kunapi) y Minthostachys cf. mollis (kunth) Griseb (Kurarina) sobre el veneno de Bothrops atrox (“Pitalala”) |
| title_full | Actividad alexitera de Tabernaemontana sananho Ruiz & Pav. (Kunapi) y Minthostachys cf. mollis (kunth) Griseb (Kurarina) sobre el veneno de Bothrops atrox (“Pitalala”) |
| title_fullStr | Actividad alexitera de Tabernaemontana sananho Ruiz & Pav. (Kunapi) y Minthostachys cf. mollis (kunth) Griseb (Kurarina) sobre el veneno de Bothrops atrox (“Pitalala”) |
| title_full_unstemmed | Actividad alexitera de Tabernaemontana sananho Ruiz & Pav. (Kunapi) y Minthostachys cf. mollis (kunth) Griseb (Kurarina) sobre el veneno de Bothrops atrox (“Pitalala”) |
| title_short | Actividad alexitera de Tabernaemontana sananho Ruiz & Pav. (Kunapi) y Minthostachys cf. mollis (kunth) Griseb (Kurarina) sobre el veneno de Bothrops atrox (“Pitalala”) |
| title_sort | Actividad alexitera de Tabernaemontana sananho Ruiz & Pav. (Kunapi) y Minthostachys cf. mollis (kunth) Griseb (Kurarina) sobre el veneno de Bothrops atrox (“Pitalala”) |
| topic | BIOTECNOLOGÍA ACTIVIDAD ALEXÍTERA TABERNAEMONTANA SANANHO BOTHORPS ATROX |
| url | http://dspace.ups.edu.ec/handle/123456789/14639 |