Evaluación in vitro de la actividad inhibitoria de aceites esenciales y sus mezclas (Curcuma longa, Cymbopogon citratus, ocotea quixos, melaleuca armillaris, zingiber officinale) en aeromona hydrophila, aeromona salmonicida y Pseudomona fluorescens
The use of antibiotics in the aquaculture industry has brought problems of bacterial and environmental resistance, facts that have forced to look for natural alternatives for the treatment of diseases produced by bacteria, being an important option the essential oils given their potential antimicrob...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | |
| التنسيق: | bachelorThesis |
| اللغة: | spa |
| منشور في: |
2018
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://dspace.ups.edu.ec/handle/123456789/15790 |
| الوسوم: |
إضافة وسم
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إضافة وسم | _version_ | 1863428385725743104 |
|---|---|
| author | Ortega Mayorga, Cristhian Alexander |
| author2 | Usca Narváez, Pamela de los Ángeles |
| author2_role | author |
| author_facet | Ortega Mayorga, Cristhian Alexander Usca Narváez, Pamela de los Ángeles |
| author_role | author |
| collection | Repositorio Universidad Politécnica Salesiana |
| dc.contributor.none.fl_str_mv | Larenas Uría, Christian Fabricio |
| dc.creator.none.fl_str_mv | Ortega Mayorga, Cristhian Alexander Usca Narváez, Pamela de los Ángeles |
| dc.date.none.fl_str_mv | 2018-07-25T20:47:44Z 2018-07-25T20:47:44Z 2018-07 |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dspace.ups.edu.ec/handle/123456789/15790 |
| dc.language.none.fl_str_mv | spa |
| dc.rights.none.fl_str_mv | Atribución-NoComercial-SinDerivadas 3.0 Ecuador http://creativecommons.org/licenses/by-nc-nd/3.0/ec/ info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Politécnica Salesiana instname:Universidad Politécnica Salesiana instacron:UPS |
| dc.subject.none.fl_str_mv | BIOTECNOLOGÍA DE LOS RECURSOS NATURALES ACEITES ESENCIALES CURCUMA LONGA CYMBOPOGON ZINGIBER |
| dc.title.none.fl_str_mv | Evaluación in vitro de la actividad inhibitoria de aceites esenciales y sus mezclas (Curcuma longa, Cymbopogon citratus, ocotea quixos, melaleuca armillaris, zingiber officinale) en aeromona hydrophila, aeromona salmonicida y Pseudomona fluorescens |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bachelorThesis |
| description | The use of antibiotics in the aquaculture industry has brought problems of bacterial and environmental resistance, facts that have forced to look for natural alternatives for the treatment of diseases produced by bacteria, being an important option the essential oils given their potential antimicrobial characteristics that help to develop sustainable aquaculture. In the research, the minimum inhibitory concentration was determined by the technique of microdilution of essential oils (Curcuma longa, Cymbopogon citratus, Ocotea quixos, Melaleuca armillaris and Zingiber officinale) and their binary and ternary mixtures on three common bacteria that cause diseases in water fish. The analysis of the composition of the essential oils was carried out by gas chromatography coupled to masses (GC-MS), being able to determine the percentage composition of each component of the oils analyzed. For Aeromona salmonicida a MIC of 0.075 mg / mL was obtained, which corresponds to Cymbopogon citratus classified as a strong activity treatment. In Aeromona hydrophila a MIC of 1.25 mg / mL classified as a moderate activity corresponding to the mixture of oils: Cymbopogon citratus and Ocotea quixos. For Pseudomonas fluorescens, a MIC of 5mg / mL was determined, corresponding to moderate activity in the mixture of Curcuma longa, Ocotea quixos and Zingiber officinale oils. It was determined that the majority of mixed oils had a partial synergism and effect of addition, only the mixture Zingiber officinale, Ocotea quixos and Melaleuca armillaris showed total synergism. |
| eu_rights_str_mv | openAccess |
| format | bachelorThesis |
| id | UPS_df85e50cc164f0906ee29ed79205a149 |
| instacron_str | UPS |
| institution | UPS |
| instname_str | Universidad Politécnica Salesiana |
| language | spa |
| network_acronym_str | UPS |
| network_name_str | Repositorio Universidad Politécnica Salesiana |
| oai_identifier_str | oai:dspace.ups.edu.ec:123456789/15790 |
| publishDate | 2018 |
| reponame_str | Repositorio Universidad Politécnica Salesiana |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Politécnica Salesiana - Universidad Politécnica Salesiana |
| repository_id_str | 1737 |
| rights_invalid_str_mv | Atribución-NoComercial-SinDerivadas 3.0 Ecuador http://creativecommons.org/licenses/by-nc-nd/3.0/ec/ |
| spelling | Evaluación in vitro de la actividad inhibitoria de aceites esenciales y sus mezclas (Curcuma longa, Cymbopogon citratus, ocotea quixos, melaleuca armillaris, zingiber officinale) en aeromona hydrophila, aeromona salmonicida y Pseudomona fluorescensOrtega Mayorga, Cristhian AlexanderUsca Narváez, Pamela de los ÁngelesBIOTECNOLOGÍA DE LOS RECURSOS NATURALESACEITES ESENCIALESCURCUMA LONGACYMBOPOGONZINGIBERThe use of antibiotics in the aquaculture industry has brought problems of bacterial and environmental resistance, facts that have forced to look for natural alternatives for the treatment of diseases produced by bacteria, being an important option the essential oils given their potential antimicrobial characteristics that help to develop sustainable aquaculture. In the research, the minimum inhibitory concentration was determined by the technique of microdilution of essential oils (Curcuma longa, Cymbopogon citratus, Ocotea quixos, Melaleuca armillaris and Zingiber officinale) and their binary and ternary mixtures on three common bacteria that cause diseases in water fish. The analysis of the composition of the essential oils was carried out by gas chromatography coupled to masses (GC-MS), being able to determine the percentage composition of each component of the oils analyzed. For Aeromona salmonicida a MIC of 0.075 mg / mL was obtained, which corresponds to Cymbopogon citratus classified as a strong activity treatment. In Aeromona hydrophila a MIC of 1.25 mg / mL classified as a moderate activity corresponding to the mixture of oils: Cymbopogon citratus and Ocotea quixos. For Pseudomonas fluorescens, a MIC of 5mg / mL was determined, corresponding to moderate activity in the mixture of Curcuma longa, Ocotea quixos and Zingiber officinale oils. It was determined that the majority of mixed oils had a partial synergism and effect of addition, only the mixture Zingiber officinale, Ocotea quixos and Melaleuca armillaris showed total synergism.El uso de antibióticos en la industria acuícola ha traído problemas de resistencia bacteriana y ambientales, hechos que ha obligado a buscar alternativas naturales para el tratamiento de enfermedades producidas por bacterias, siendo una importante opción los aceites esenciales dadas sus potenciales características antimicrobianas que ayuden a desarrollar una acuicultura sustentable. En la investigación se determinó la Concentración mínima inhibitoria mediante la técnica de microdilución de aceites esenciales (Curcuma longa, Cymbopogon citratus, Ocotea quixos, Melaleuca armillaris y Zingiber officinale) y sus mezclas binarias y ternarias sobre tres bacterias comunes que causan enfermedades en peces de agua dulce. El análisis de la composición de los aceites esenciales se realizó por medio de cromatografía de gases acoplado a masas (GC-MS), pudiendo determinar la composición porcentual de cada componente de los aceites analizados. Para Aeromona salmonicida se obtuvo un MIC de 0.075 mg/mL que corresponde a Cymbopogon citratus clasificado como tratamiento de actividad fuerte. En Aeromona hydrophila un MIC de 1.25 mg/mL clasificada como una actividad moderada correspondiente a la mezcla de aceites: Cymbopogon citratus y Ocotea quixos. Para Pseudomona fluorescens se determinó un MIC de 5mg/mL correspondiendo a una actividad moderada a la mezcla de aceites Curcuma longa, Ocotea quixos y Zingiber officinale. Se determinó que la mayoría de aceites mezclados tuvieron un sinergismo parcial y efecto de adición, solo la mezcla Zingiber officinale, Ocotea quixos y Melaleuca armillaris mostro sinergismo total.Larenas Uría, Christian Fabricio2018-07-25T20:47:44Z2018-07-25T20:47:44Z2018-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttp://dspace.ups.edu.ec/handle/123456789/15790spaAtribución-NoComercial-SinDerivadas 3.0 Ecuadorhttp://creativecommons.org/licenses/by-nc-nd/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Politécnica Salesianainstname:Universidad Politécnica Salesianainstacron:UPS2019-01-01T19:13:01Zoai:dspace.ups.edu.ec:123456789/15790Institucionalhttps://dspace.ups.edu.ec/Institución privadahttps://www.ups.edu.ec/https://dspace.ups.edu.ec/oai.Ecuador...opendoar:17372019-01-01T19:13:01Repositorio Universidad Politécnica Salesiana - Universidad Politécnica Salesianafalse |
| spellingShingle | Evaluación in vitro de la actividad inhibitoria de aceites esenciales y sus mezclas (Curcuma longa, Cymbopogon citratus, ocotea quixos, melaleuca armillaris, zingiber officinale) en aeromona hydrophila, aeromona salmonicida y Pseudomona fluorescens Ortega Mayorga, Cristhian Alexander BIOTECNOLOGÍA DE LOS RECURSOS NATURALES ACEITES ESENCIALES CURCUMA LONGA CYMBOPOGON ZINGIBER |
| status_str | publishedVersion |
| title | Evaluación in vitro de la actividad inhibitoria de aceites esenciales y sus mezclas (Curcuma longa, Cymbopogon citratus, ocotea quixos, melaleuca armillaris, zingiber officinale) en aeromona hydrophila, aeromona salmonicida y Pseudomona fluorescens |
| title_full | Evaluación in vitro de la actividad inhibitoria de aceites esenciales y sus mezclas (Curcuma longa, Cymbopogon citratus, ocotea quixos, melaleuca armillaris, zingiber officinale) en aeromona hydrophila, aeromona salmonicida y Pseudomona fluorescens |
| title_fullStr | Evaluación in vitro de la actividad inhibitoria de aceites esenciales y sus mezclas (Curcuma longa, Cymbopogon citratus, ocotea quixos, melaleuca armillaris, zingiber officinale) en aeromona hydrophila, aeromona salmonicida y Pseudomona fluorescens |
| title_full_unstemmed | Evaluación in vitro de la actividad inhibitoria de aceites esenciales y sus mezclas (Curcuma longa, Cymbopogon citratus, ocotea quixos, melaleuca armillaris, zingiber officinale) en aeromona hydrophila, aeromona salmonicida y Pseudomona fluorescens |
| title_short | Evaluación in vitro de la actividad inhibitoria de aceites esenciales y sus mezclas (Curcuma longa, Cymbopogon citratus, ocotea quixos, melaleuca armillaris, zingiber officinale) en aeromona hydrophila, aeromona salmonicida y Pseudomona fluorescens |
| title_sort | Evaluación in vitro de la actividad inhibitoria de aceites esenciales y sus mezclas (Curcuma longa, Cymbopogon citratus, ocotea quixos, melaleuca armillaris, zingiber officinale) en aeromona hydrophila, aeromona salmonicida y Pseudomona fluorescens |
| topic | BIOTECNOLOGÍA DE LOS RECURSOS NATURALES ACEITES ESENCIALES CURCUMA LONGA CYMBOPOGON ZINGIBER |
| url | http://dspace.ups.edu.ec/handle/123456789/15790 |