Estrategias terapéuticas de aceites esenciales microencapsulados en escherichia coli atcc 25922, pseudomonas aeruginosa atcc 10145, staphylococcus aureus atcc 25923 y candida albicans atcc 10231.
According to the World Health Organization (WHO), there has been an 82% increase in antibiotic-resistant infections since 2018 (1). This increase is linked to the overuse of antimicrobials, which is the primary cause of the existence of resistant and multiresistant strains (2). For this reason, we p...
-д хадгалсан:
| Үндсэн зохиолч: | |
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| Формат: | masterThesis |
| Хэл сонгох: | spa |
| Хэвлэсэн: |
2024
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| Нөхцлүүд: | |
| Онлайн хандалт: | https://repositorio.uta.edu.ec/handle/123456789/42946 |
| Шошгууд: |
Шошго нэмэх
Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!
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Шошго нэмэх | Тойм: | According to the World Health Organization (WHO), there has been an 82% increase in antibiotic-resistant infections since 2018 (1). This increase is linked to the overuse of antimicrobials, which is the primary cause of the existence of resistant and multiresistant strains (2). For this reason, we propose using microencapsulated essential oils (MEA) as therapeutic strategies. To evaluate the antimicrobial effect of the EMPs of Anethum graveolens (eneldo), Rosmarinus officinalis (romero), Mentha spicata (menta), Ocimum basilicum (albahaca) and Cymbopogon citratus (hierba luisa) on Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 10145, Staphylococcus aureus ATCC 25923 and Candida albicans ATCC 10231. The microencapsulation of essential oils (EO) was carried out with the particle spray drying technique using the Mini Spray Dryer Büchi B-290 equipment, encapsulating polymers were used in a ratio 80:20 (%v/v). For the evaluation of the antimicrobial properties of AEM, the modified Kirby-Baüer method was used. In addition, the minimum inhibitory concentration (CIM) and the minimum bactericidal concentration (CMB) of the AEM were determined by the viability technique with 0.1% resazurin. In the microencapsulation process of EOs, their effectiveness was demonstrated in the wavelength ranges from 1604 to 1112 cm-1 obtained from the infrared ATR-FTIR, Nicolet IR100. Regarding the antimicrobial effect produced by the AEM, it was obtained that Escherichia coli ATCC 25922 was inhibited with the Ocimum basilicum AEM, generating inhibition zones of 17.5 mm, a (CIM) of 0.0075g and a (CMB) of 0.03 g. On the other hand, in Staphylococcus aureus ATCC 25923 the AEM of Rosmarinus officinalis and Cymbopogon citratus presented inhibition zones of 14 mm, 16 mm, a CIM of 0.0075g and CMB of 0.03g respectively. Concerning Pseudomonas aeruginosa ATCC 27853, inhibition was observed with the AEM Cymbopogon citratus producing inhibition zones of 19 mm, an CIM of 0.015g and the CMB of 0.03g. In the analysis of Candida albicans ATCC 10231, an antifungal effect was observed with all AEMs. The EMPs of Anethum graveolens, Rosmarinus officinalis, Mentha spicata, Ocimum basilicum, and Cymbopogon citratus in ATCC microorganisms presented antimicrobial activity. |
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