MUTAGÉNESIS INDUCIDA EN Eriochloa polystachya MEDIANTE ETIL-METASULFONATO Y SU EFECTO SOBRE EL CONTROL DE Mahanarva andigena BAJO CONDICIONES DE INVERNADERO

Through genetic improvement, improved plant species can be acquired, with induced mutagenesis being one of the techniques used, for which ethyl methane sulfonate is used as a chemical agent. The janeiro grass Eriochloa polystachya belongs to the Poaceas, distributed throughout South America with high nutritional value and of great importance for livestock feeding, in the same way, it is susceptible to insect pests such as Spittlebug. Therefore, the objective of this research project was to evaluate the effect of Ethyl-Methosulfonate in E. polystachya to the attack of Spittlebug under greenhouse conditions. The experimental design methodology was used, the grass was sown by stolons in germinating trays to determine the LD 50. The information was analyzed through the Complete Random Design, with Probit linear regression in ten treatments and three repetitions, where the percentage variable of mortality presented high significance. Then the agronomic behavior presented by the janeiro grass impregnated with the different doses and at different times was evaluated, for this, data such as plant height, stem diameter, number of leaves, width and length of the leaf were taken, the information was analyzed at through InfoStat, which presented high significance. Finally, Janeiro grass plants with characteristics of resistance to spittle attack were selected, for which descriptors were used that allowed us to record the number of spittlebugs, severity and growth vigor. The results obtained showed in the linear regression that the LD 50 at 24 hours was adjusted to a dose of 0.49% EMS while at 48 hours it was 0.32%, presenting a better R2 = 79.62. On the other hand, in the agronomic behavior, it was evidenced that T3 with doses of 0.50% and at 24 hours together with T7 with doses of 0.25% at 48 hours, presented high significance and were statistically superior to the other treatments under study. Regarding the selection of janeiro grass tolerant to the attack of Spittlebug, it was observed that the treatment T5 and T10 whose doses were at 1% concentration of EMS, showed a lower number of spittlebugs present, therefore, they were treatments that did not present any damage. In contrast, treatments with a lower dose of EMS showed mild and moderate damage, with spittlebug populations of less than 20 individuals.

• Glavni avtor: Cortez Herrera, Isrrael (author)
• Format: masterThesis
• Izdano: 2021
• Teme: Mutagénesis; Etil-metanosulfonato; Pasto Janeiro; Invernadero
• Online dostop: http://dspace.utb.edu.ec/handle/49000/10070