Preñez de vacas mestizas con la implantación de embriones in vivo e in vitro, para mejorar la genética bovina en Macas

This research aimed to evaluate the effectiveness of multi-ovulation and embryo transfer (MOET) and in vitro embryo production (PIVE) on embryo production and pregnancy rate. The study was conducted at Leone's farm in Macas Canton, Morona Santiago Province, Ecuador. Three Charol donors were used for each technique and 40 recipient cows (30 for live embryos and 10 for in vitro embryos). For MOET, a superovulation treatment combined with an ovulation synchronization treatment was applied. Oestrus was detected, and cows were inseminated with two doses of semen. On day seven after oestrus, uterine lavage was performed, and grade 1 embryos were transferred to recipient cows. Oocyte donor cows were collected under epidural anesthesia using the ultrasound-guided follicular aspiration method. Embryos produced were transferred fresh on day seven after in vitro fertilization. Data were analyzed by descriptive statistics using SAS software. MOET donor cows produced 30 grade 1 transferable embryos, which produced 16 pregnancies (pregnancy rate of 53.1 ± 7.2%). From the three donor cows used for PIVE, 25 oocytes were collected, of which 10 reached the blastocyst stage and were transferred, resulting in only one pregnant cow (pregnancy rate of 20 ± 17.3%). The economic analysis showed that the cost per embryo with MOET was 2.5 times lower than with PIVE (195.7 and 500.0 US$ respectively). It is concluded that MOET was the most effective reproductive bio-technique, both biologically and economically, for the production of embryos for breeding purposes.

• Autor principal: Pilla Caizabanda, Nina Mónica (author)
• Formato: bachelorThesis
• Lenguaje: spa
• Publicado: 2021
• Materias: TRANSFERENCIA DE EMBRIONES; PRODUCCIÓN DE EMBRIONES; TRATAMIENTO SUPEROVULATORIO; MEDICINA VETERINARIA
• Acceso en línea: http://repositorio.utc.edu.ec/handle/27000/7913