Comparación de las técnicas qPCR vs kits rápidos en el Diagnóstico de Leucemia Felina (ViLeF) en el Centro de Diagnóstico Veterinario VETNAAT en la ciudad de Quito.
Feline leukemia is one of the main viral diseases affecting domestic and/or wild felines due to the fact that it has several forms of transmission, among which we find: oral, nasal, sexual, transplacental and intrauterine. For its diagnosis different tests are used such as: ELISA or rapid kits, real...
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| Format: | bachelorThesis |
| Language: | spa |
| Published: |
2022
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| Subjects: | |
| Online Access: | http://repositorio.utc.edu.ec/handle/27000/9716 |
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Add Tag | Summary: | Feline leukemia is one of the main viral diseases affecting domestic and/or wild felines due to the fact that it has several forms of transmission, among which we find: oral, nasal, sexual, transplacental and intrauterine. For its diagnosis different tests are used such as: ELISA or rapid kits, real-time PCR, Rt-qPCR, immunoassay, immucromatography, among others; of which the most used at clinical level is the ELISA test or rapid kits. For this reason, the objective of the current research project is to compare the sensitivity and specificity of the rapid kits vs. the qPCR molecular test, as well as to analyze the prevalence of risk factors. For this purpose, 26 felines positive or suspicious for Feline Leukemia were used; of which 16 were females and 10 males of different ages and reproductive status; blood samples were collected using the cephalic vein for the rapid kits, and blood plasma for the qPCR molecular test from which the viral RNA was extracted and amplified at the VetNAAT Veterinary Diagnostic Center. For the results, 4 risk factors were taken into consideration, the most important of which are the sex risk factor, with a prevalence of 11.54% for infected males and 15.38% for infected females, and the vaccination status risk factor, with a prevalence of 8.55% for unvaccinated felines and 17.31% for vaccinated felines. According to the data obtained, the disease has no predilection for sex or vaccination status of the felines; qPCR is the most accurate diagnostic technique for the detection of Feline Leukemia (ViLeF), and its results are more reliable. The rapid tests normally used for the diagnosis of the disease in this study have shown low sensitivity. |
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