Evaluación de los Niveles de Progesterona en Vacas donadoras Superovuladas en relación a la Calidad Embrionaria

Among the main factors that determine the success of a multiple ovulation program and embryo transfer (MOET) is the high variability in the superovulatory response linked to the race, age, type, altitude and dose of FSH. Thus, some studies show that the plasma concentration of progesterone (P4) finds a relationship between embryonic production and P4 concentration, but others do not. The aim of the present work research was to study the relationship of plasma progesterone concentration with embryonic viability and quality to the day of embryo collection in the donor. They were selected and superovulation with follicle-stimulating hormone (FSH-P) at decreasing doses, 7 female bovine Holstein Friesian breed ranging from 2 to 10 years of age. The embryo collection was carried out 7 days after the first insemination, as well as taking blood samples to determine P4 by ELISA. The data was analyzed using the t Student test. It is determined that the levels of plasma progesterone in donors of superovulated embryos at day 8 post insemination range from 1.67ng / ml, 2.05 and 67.32 ng / ml, in addition the stages of development of morulas, Early blastocysts and blastocysts are corresponding to the progressive ovulation events of the donor; thus, levels of progesterone concentration higher than 20ng / ml established an adequate embryonic development and its maintenance in grade 1 (excellent), at lower levels than 15 ng / ml, determined a decrease in embryonic development and degeneration. It is concluded that embryonic viability (morphology - grade I, II and III classification) has a positive correlation with plasma progesterone levels in superovulated donor cows

• Autor Principal: Ramírez Balseca, Danny Israel (author)
• Formato: bachelorThesis
• Idioma: spa
• Publicado: 2018
• Subjects: SUPEROVULACIÓN; PROGESTERONA; EMBRIONES
• Acceso en liña: http://repositorio.utc.edu.ec/handle/27000/5214