Identificación molecular del ADN de Toxocara canis.
The present research was carried out from October 2020 to March 2021 in Cotopaxi province, Latacunga City, in the rural areas of the city, the objective of this research is the molecular identification of Toxocara canis DNA. We collected fecal matter samples in 150 domestic canines, male and female...
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| Format: | bachelorThesis |
| Język: | spa |
| Wydane: |
2021
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| Hasła przedmiotowe: | |
| Dostęp online: | http://repositorio.utc.edu.ec/handle/27000/10227 |
| Etykiety: |
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Dodaj etykietę | Streszczenie: | The present research was carried out from October 2020 to March 2021 in Cotopaxi province, Latacunga City, in the rural areas of the city, the objective of this research is the molecular identification of Toxocara canis DNA. We collected fecal matter samples in 150 domestic canines, male and female dogs, of the registered owners, to the performance of the deworming tests and to evaluate the presence of Toxocara canis gastrointestinal parasite eggs. The age of the canines under research is from 2 months to 3 years, prioritizing the deworming tests in the population in the age from 2-9 months old. We performed the respective analysis of the sample using Sheather's flotation technique, which involved taking 3 grams of the sample and homogenizing it in 20 grams of Sheather's solution. According to the microscopic examination 16 samples gave positive for the presence of Toxocara canis parasite eggs. The entire population under study was dewormed orally due to the fact that the canines are located in rural areas of the city, therefore they do not have adequate prophylactic and sanitary care of domestic animals, 25 adult specimens of Toxocara canis were obtained in total and 16 were identified by deworming tests technique, based on macroscopic diagnoses; the remaining 9 specimens were obtained without deworming tests due to the fact that the progenitor of the specimens had a parasitic veterinary history. 100% of these parasites were identified as Toxocara canis based on morphological characteristics, it was determined that the microscopic technique is not effective when examining a low parasite load. We follow the dissection protocols for the Ascaridae family for the morphological typing. Of the 20 adult specimens of Toxocara canis evaluated, 19 were confirmed by PCR test, 1 sample was negative since there were several factors that have already influenced the result of the sample. |
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