Elaboración de un Protocolo y Metodología de Recoleccion de Especies de Hongos en la estructura del Sustrato Vegetal del Bosque Siempre Verde Montano(bsmn03) de la Cordillera Occidental de Los Andes en el Cantón Pujilí, Parroquia El Tingo La Esperanza en el período 2019 – 2020.

This research was carried out through an analysis of scientific bibliographic information to carry out a protocol and methodology for collecting fungal species in the structure of the plant substrate of the Evergreen Montane forest (BsMn03) at western mountain range of the Andes in El Tingo - La Esperanza parish from Pujilí canton. The study area is located in an altitudinal range from 2310 to 2360 masl with UTM coordinates for point 1 X: 715479 and Y: 9894927 and for point 2 X: 717220 and Y: 9894015, it is located at northwestern part of the canton. The main objective was to carry out a study to prepare the methodology and protocol for fungal species collection present in plant substrate in the field and laboratory phases. The type of research was descriptive supported by documentary bibliographic research that allowed collecting scientific written information with theoretical basis and it was a support to the researched results. To carry out soil sampling, the study area is georeferenced, then it is divided into 10 homogeneous transects of 100 m2 and a stratified random sampling is carried out every 250 m of height, 5 samples were taken in each transect with a hole up to a 0.2 m deep, they are packed in plastic bags duly labeled with the exact information of the place. Once in the laboratory, the samples, materials and equipment are disinfected, the samples are sieved on a 2mm sieve and then sterilized with an autoclave for 15 minutes at 20 ° C; a solution of 10 grams of soil is made in 90 mL of distilled water, it proceeds to make five dilutions of the soil sample in base 10. The culture medium is prepared, potato dextrose agar using 200 grams of unpeeled potato, 10 grams of dextrose, 18 grams of agar and 1 liter of distilled water, boil the water and the potatoes and proceed to strain, place the dextrose and the agar and stir, the solution is sterilized for 15 minutes in a autoclave. Once the culture medium is ready, in a laminar flow chamber it is placed in Petri dishes, then the dilutions made are sown by taking with a handle and by means of a simple touch or by means a continuous scratching, the boxes are sealed and incubate for 3 to 6 days at 25 ° C. Later the incubated plates meet the proliferation of fungi mycelium, after that microscopic observation was made using the adhesive tape technique, sample was extracted from mycelium and place it on the slide plate applying a solution of blue methylene to color fungal structures. Notes were taken of the morphological structures and with the help of fungal identification keys, they are identified.

• 主要作者: Padilla Sangucho, Jessica Maribel (author)
• 格式: bachelorThesis
• 语言: spa
• 出版: 2020
• 主题: SUELO; ESPECIES; HONGOS; BOSQUE SIEMPRE VERDE MONTAN; MEDIO AMBIENTE
• 在线阅读: http://repositorio.utc.edu.ec/handle/27000/7096