Identificación de agentes bacterianos en cuyes (cavia porcellus) con signos respiratorios en el sector Santan del cantón Latacunga
Guinea pig breeding has acquired significant economic and social relevance in several Latin American countries, especially in smaller production systems. Despite advances in disciplines such as nutrition, animal management and genetic improvement, animal health has received less attention in researc...
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| Format: | bachelorThesis |
| Idioma: | spa |
| Publicat: |
2024
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| Matèries: | |
| Accés en línia: | http://repositorio.utc.edu.ec/handle/27000/12139 |
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Afegir etiqueta | Sumari: | Guinea pig breeding has acquired significant economic and social relevance in several Latin American countries, especially in smaller production systems. Despite advances in disciplines such as nutrition, animal management and genetic improvement, animal health has received less attention in research coordinated by experimental centres. Although progress has been made in several areas, it is crucial to address health and biosecurity deficiencies to ensure sustainable growth in guinea pig breeding and facilitate expansion into international markets. Given the susceptibility of guinea pigs to respiratory tract infections, whether bacterial or viral, several productions have been affected. Therefore, research was carried out to identify the bacterial agents responsible for respiratory problems in guinea pigs (Cavia porcellus). Microbiological techniques were applied to carry out a morphological analysis of the bacterial species present in five producers in the Santan sector in the canton of Latacunga, where two producers with several guinea pigs with respiratory infections were identified. Samples of 20 affected organs were collected from these animals. Lungs, trachea, heart, liver and swollen lymph nodes. A protocol involving the analysis of both Gram-negative bacteria with Mac Conkey's Selective Agar and Gram-positive bacteria with Blood Agar was implemented. Different enrichment procedures were carried out and specific culture media were used to examine bacterial morphology and behaviour with biochemical tests. A remarkable percentage of gram-negative bacteria was identified, mainly from the Enterobacteriae family, as 24.32% were observed with the first protocol, and 20.45% with the second protocol. The predominant species in the first protocol for gram-negative bacteria was Klebsiella spp. with 27.03%, a common enterobacterium in these animals. In the protocol for gram-positive bacteria, a high percentage of Streptococcus spp. of 27.27% was observed. Given the abundance of bacteria analysed, it is recommended to continue with the bacterial isolation process for specificity, to use selective media, as well as to perform other specific biochemical tests, taking into consideration the antibiogram analysis for the microorganisms identified in the following procedures of this study. |
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