Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático.

ABSTRACT This research objective was to evaluate the addition of an enzyme preparation in which a 1:1 dilution of chontaduro masate and water as solvent was made, where the concentrations of the enzymes α-amylase and β-amylase were calculated, which was added to the solution, it was evaluated during...

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Päätekijä: Villacís Acosta, Daysi Marisol (author)
Muut tekijät: Villacís Tubón, Enma Gabriela (author)
Aineistotyyppi: bachelorThesis
Kieli:spa
Julkaistu: 2021
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Linkit:http://repositorio.utc.edu.ec/handle/27000/10136
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author Villacís Acosta, Daysi Marisol
author2 Villacís Tubón, Enma Gabriela
author2_role author
author_facet Villacís Acosta, Daysi Marisol
Villacís Tubón, Enma Gabriela
author_role author
collection Repositorio Universidad Técnica de Cotopaxi
dc.contributor.none.fl_str_mv Arias Palma, Gabriela Beatriz
dc.creator.none.fl_str_mv Villacís Acosta, Daysi Marisol
Villacís Tubón, Enma Gabriela
dc.date.none.fl_str_mv 2021-03
2023-04-27T16:24:44Z
2023-04-27T16:24:44Z
dc.format.none.fl_str_mv 153 páginas
dc.identifier.none.fl_str_mv Villacís Acosta Daysi Marisol, Villacís Tubón Enma Gabriela (2021); Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático. UTC. Latacunga. 153 p.
PC-002593
http://repositorio.utc.edu.ec/handle/27000/10136
dc.language.none.fl_str_mv spa
dc.publisher.none.fl_str_mv Ecuador: Latacunga (Universidad Técnica de Cotopaxi)
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/ec/
info:eu-repo/semantics/openAccess
dc.source.none.fl_str_mv reponame:Repositorio Universidad Técnica de Cotopaxi
instname:Universidad Técnica de Cotopaxi
instacron:UTC
dc.subject.none.fl_str_mv CHONTADURO
Α-AMILASA
Β-AMILASA
AGROINDUSTRIAL
dc.title.none.fl_str_mv Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático.
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/bachelorThesis
description ABSTRACT This research objective was to evaluate the addition of an enzyme preparation in which a 1:1 dilution of chontaduro masate and water as solvent was made, where the concentrations of the enzymes α-amylase and β-amylase were calculated, which was added to the solution, it was evaluated during the time of 72 hours, where the treatments were at room temperature, for the chonta drink fermentation, the enzymatic preparation was used as a substitute for chewing, since, by hand, this Chicha is fermented thanks to the amylase contained in people's saliva. For which a completely randomized block design (DBCA) was performed, with factorial arrangement AxB (3x2), with 2 repetitions, obtaining a total of 18 treatments, the factors evaluated were: factor A (concentration of enzyme preparation) and factor B (activation time), 6 treatments were formulated as follows: t1 (0.05% - 40 min), t2 (0.05% -80 min), t3 (0.10% - 40 min), t4 (0.10% - 80 min), t5 (0.15% - 40 min), t6 (0.15% - 80 min) and the fermentation process was evaluated according to the following response variables: pH, soluble solids and acidity; data that were taken every 24 hours, from 0 hours to 72 hours. The statistical analysis of the data obtained was carried out using the Infostat software, with which the best treatment t6 a3b2 (0.15% - 0.80 min) was determined, presenting a pH of 4.86 and titratable acidity of 0.44 %, Brix degrees of 8.47, for which it was determined as the best treatment in the fermentation process. After analyzing the best treatment in the fermentation process of the chonta drink (Bactris gasipaes), the stability of the best treatment was evaluated by applying the same fermentation preparation methodology until the stabilization process was reached, where the stabilizers xanthan gum and carboxymethylcellulose. For which a completely randomized block design (DBCA) was carried out, under a factorial arrangement AxBxC (2x2x2), with two repetitions, obtaining a total of 16 treatments, where the factors were: factor A (type of stabilizer), factor B (percentage of stabilizer), factor C (storage temperature), 8 treatments were formulated as follows: t1 (xanthan gum - 0.30% - refrigeration), t2 (xanthan gum - 0.15% - refrigeration), t3 (xanthan gum - 0.30% - room temperature), t4 (xanthan gum - 0.15% - room temperature), t5 (CMC - 0.30% - refrigeration), t6 (CMC - 0.15% - refrigeration), t7 (CMC - 0.30% - ambient temperature), t8 (CMC - 0.15% - ambient temperature) and the stabilization process was evaluated according to the following response variables: syneresis, density and viscosity, the data were taken every 24 hours for three days, using the statistical program Infostat, through which it was possible to determine that the best treatment was t2 a1b2c1 (xanthan gum a - 0.15% - refrigeration), presenting a syneresis of 0%, a density of 1.01 and a viscosity of 0.38 poises, for which the appropriate treatment was determined in comparison to the other treatments.
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identifier_str_mv Villacís Acosta Daysi Marisol, Villacís Tubón Enma Gabriela (2021); Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático. UTC. Latacunga. 153 p.
PC-002593
instacron_str UTC
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instname_str Universidad Técnica de Cotopaxi
language spa
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oai_identifier_str oai:oai:repositorio.utc.edu.ec:27000:27000/10136
publishDate 2021
publisher.none.fl_str_mv Ecuador: Latacunga (Universidad Técnica de Cotopaxi)
reponame_str Repositorio Universidad Técnica de Cotopaxi
repository.mail.fl_str_mv .
repository.name.fl_str_mv Repositorio Universidad Técnica de Cotopaxi - Universidad Técnica de Cotopaxi
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rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/ec/
spelling Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático.Villacís Acosta, Daysi MarisolVillacís Tubón, Enma GabrielaCHONTADUROΑ-AMILASAΒ-AMILASAAGROINDUSTRIALABSTRACT This research objective was to evaluate the addition of an enzyme preparation in which a 1:1 dilution of chontaduro masate and water as solvent was made, where the concentrations of the enzymes α-amylase and β-amylase were calculated, which was added to the solution, it was evaluated during the time of 72 hours, where the treatments were at room temperature, for the chonta drink fermentation, the enzymatic preparation was used as a substitute for chewing, since, by hand, this Chicha is fermented thanks to the amylase contained in people's saliva. For which a completely randomized block design (DBCA) was performed, with factorial arrangement AxB (3x2), with 2 repetitions, obtaining a total of 18 treatments, the factors evaluated were: factor A (concentration of enzyme preparation) and factor B (activation time), 6 treatments were formulated as follows: t1 (0.05% - 40 min), t2 (0.05% -80 min), t3 (0.10% - 40 min), t4 (0.10% - 80 min), t5 (0.15% - 40 min), t6 (0.15% - 80 min) and the fermentation process was evaluated according to the following response variables: pH, soluble solids and acidity; data that were taken every 24 hours, from 0 hours to 72 hours. The statistical analysis of the data obtained was carried out using the Infostat software, with which the best treatment t6 a3b2 (0.15% - 0.80 min) was determined, presenting a pH of 4.86 and titratable acidity of 0.44 %, Brix degrees of 8.47, for which it was determined as the best treatment in the fermentation process. After analyzing the best treatment in the fermentation process of the chonta drink (Bactris gasipaes), the stability of the best treatment was evaluated by applying the same fermentation preparation methodology until the stabilization process was reached, where the stabilizers xanthan gum and carboxymethylcellulose. For which a completely randomized block design (DBCA) was carried out, under a factorial arrangement AxBxC (2x2x2), with two repetitions, obtaining a total of 16 treatments, where the factors were: factor A (type of stabilizer), factor B (percentage of stabilizer), factor C (storage temperature), 8 treatments were formulated as follows: t1 (xanthan gum - 0.30% - refrigeration), t2 (xanthan gum - 0.15% - refrigeration), t3 (xanthan gum - 0.30% - room temperature), t4 (xanthan gum - 0.15% - room temperature), t5 (CMC - 0.30% - refrigeration), t6 (CMC - 0.15% - refrigeration), t7 (CMC - 0.30% - ambient temperature), t8 (CMC - 0.15% - ambient temperature) and the stabilization process was evaluated according to the following response variables: syneresis, density and viscosity, the data were taken every 24 hours for three days, using the statistical program Infostat, through which it was possible to determine that the best treatment was t2 a1b2c1 (xanthan gum a - 0.15% - refrigeration), presenting a syneresis of 0%, a density of 1.01 and a viscosity of 0.38 poises, for which the appropriate treatment was determined in comparison to the other treatments.La presente investigación se realizó con la finalidad de evaluar la adición de un preparado enzimático (α-amilasa y β-amilasa) en donde se realizó una dilución 1:1 de masato de chontaduro como soluto y agua como solvente, para la fermentación de la bebida de chonta se evaluó durante el tiempo de 72 horas, se utilizó el preparado enzimático como sustituto de la masticación, ya que, artesanalmente esta chicha se fermenta gracias a la amilasa que contiene la saliva de las personas, se empleó un diseño de bloques completamente al azar, con arreglo factorial AxB (3x2), con 3 repeticiones, con un total de 18 tratamientos, los factores evaluados fueron: factor A (concentración de preparado enzimático) y factor B (tiempo de activación), se evaluó el proceso de fermentación de acuerdo a las siguientes variables respuestas: pH, sólidos solubles y acidez; datos que fueron tomados desde las 0 horas hasta las 72 horas. Se empleó el programa estadístico software Infostat, mediante el cual se pudo determinar el mejor tratamiento t6 (0,15% - 0,80 min) obteniendo resultado de pH 4,86, acidez titulable de 0,44% y sólidos solubles de 8,47. Al determinar el mejor tratamiento en el proceso de fermentación de la bebida de chonta (Bactris gasipaes), se procedió a evaluar el proceso de estabilidad mediante el empleo de goma xantana y carboximetilcelulosa. Para lo cual se aplicó un diseño de bloques completamente al azar bajo un arreglo factorial AxBxC (2x2x2), con dos repeticiones, obteniendo un total de 16 tratamientos, donde los factores fueron: factor A (tipo de estabilizante), factor B (porcentaje de estabilizante), factor C (almacenamiento), este proceso fue evaluado de acuerdo a las siguientes variables respuestas: sinéresis, densidad y viscosidad, los datos fueron tomados cada 24 horas durante tres días, se utilizó el programa estadístico Infostat, determinando como el mejor tratamiento al t2 (goma xantana - 0,15% - refrigeración), presentando una sinéresis de 0%, densidad 1,01 g/cm3 y viscosidad 0,38 poises. Los resultados de análisis realizados en el laboratorio “LABOLAB” arrojaron los siguientes resultados en los análisis químicos para el pH 4,98 ± 0,13, sólidos solubles 9,70 ± 0,73, acidez 0,07, viscosidad 708,60 cP, densidad 1.0350 g/ml, azúcares 1,61%, fructosa 0,30%, lactosa 1,31% y concentración de alcohol 0,07%. Para los análisis microbiológicos se obtuvo los siguientes resultados para recuento de Aerobios totales 〖5,4x10〗^2, recuento de Coliformes totales ˂10, recuento de Mohos ˂10, recuento de Levaduras ˂10 y por último las bacterias acido lácticas 〖5,0x10〗^1.Ecuador: Latacunga (Universidad Técnica de Cotopaxi)Arias Palma, Gabriela Beatriz2023-04-27T16:24:44Z2023-04-27T16:24:44Z2021-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis153 páginasVillacís Acosta Daysi Marisol, Villacís Tubón Enma Gabriela (2021); Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático. UTC. Latacunga. 153 p.PC-002593http://repositorio.utc.edu.ec/handle/27000/10136spahttp://creativecommons.org/licenses/by-nc-sa/3.0/ec/info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Técnica de Cotopaxiinstname:Universidad Técnica de Cotopaxiinstacron:UTC2023-04-28T08:00:59Zoai:oai:repositorio.utc.edu.ec:27000:27000/10136Institucionalhttp://repositorio.utc.edu.ec/Universidad públicahttps://www.utc.edu.ec/..Ecuador...opendoar:02025-01-26T03:45:40.326849Repositorio Universidad Técnica de Cotopaxi - Universidad Técnica de Cotopaxitrue
spellingShingle Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático.
Villacís Acosta, Daysi Marisol
CHONTADURO
Α-AMILASA
Β-AMILASA
AGROINDUSTRIAL
status_str publishedVersion
title Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático.
title_full Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático.
title_fullStr Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático.
title_full_unstemmed Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático.
title_short Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático.
title_sort Evaluación de la fermentación y estabilización de una bebida de chonta (Bactris gasipaes) con preparado enzimático.
topic CHONTADURO
Α-AMILASA
Β-AMILASA
AGROINDUSTRIAL
url http://repositorio.utc.edu.ec/handle/27000/10136