Proyecto de elaboración y aplicación de un antígeno parasitario (Haemonchus) en ovinos.

This research project focused on assessing the Haemonchus parasitic antigen and its application in sheep in the Cuasualo neighborhood, in order to carry out an immunological response after inoculation, and to provide an alternative to the resulting infestation, improving the quality of life of anima...

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Bibliographic Details
Main Author: Solis Orozco, Bryan Javier (author)
Other Authors: Velasco López, Michelle Dayana (author)
Format: bachelorThesis
Language:spa
Published: 2022
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Online Access:http://repositorio.utc.edu.ec/handle/27000/9632
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Summary:This research project focused on assessing the Haemonchus parasitic antigen and its application in sheep in the Cuasualo neighborhood, in order to carry out an immunological response after inoculation, and to provide an alternative to the resulting infestation, improving the quality of life of animals while avoiding losses in economy, productivity and development of anthelmintic resistance. 30 animals were selected, and coprology tests were carried out. Haemonchus parasites were collected from the intestinal tract of animals with which the parasitic antigen was obtained and subsequently inoculated into the sheep. Blood samples were taken for immunochemical tests (Immunoglobulin E) and blood hematology (CBC). Through the flotation technique, 67% of females and 33% of males were positive for parasitosis against Haemonchus contortus, presenting a greater number of parasite eggs in the count. 47% of animals younger than 1 year presented parasitosis, while 37% between 1 and 2 years presented parasitosis, and 17% older than 2 years presented parasitosis. 83% of the subjects tested presented normal values in the values of immunoglobulin E, and 17% indicated high rates in the range of immunoglobulins. Around 83% of the animals did not present any alteration in the values of the hemogram, while 17% showed cases of anemia, hypochromia, macrocytosis and neutrophilia. Exceptionally, in the cases of leukocytosis and lymphocytosis, these values were close to 50%, processes that were determined by variations depending on the general condition of the animal against parasitosis. The inoculation of the parasitic antigen was developed and applied in order to reduce the incidence of Haemonchus in the animal population studied, as a mechanism to induce a powerful immune response, and ensure that sheep production can have control alternatives that guarantee the well-being of the sheep and the economy of the producer.