“Propagación in vitro del cultivo de mora sin espinas (Rubus glaucus benth) en el Cantón Cevallos Provincia Tungurahua”.
The blackberry has great commercial importance, it is one of the most desired and cultivated fruits in the world. The research was carried out to develop a protocol for the massive propagation via in vitro of the blackberry (Rubus glaucus Benth). From axillary buds since the fruit has been in great...
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| Formatua: | bachelorThesis |
| Hizkuntza: | spa |
| Argitaratua: |
2020
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| Sarrera elektronikoa: | http://repositorio.utc.edu.ec/handle/27000/6930 |
| Etiketak: |
Etiketa erantsi
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Etiketa erantsi | Gaia: | The blackberry has great commercial importance, it is one of the most desired and cultivated fruits in the world. The research was carried out to develop a protocol for the massive propagation via in vitro of the blackberry (Rubus glaucus Benth). From axillary buds since the fruit has been in great demand in the national and international markets. The objectives set out in this research were: to identify the appropriate starting explant to be introduced to the laboratory; to establish the appropriate conditions for the sterilization of the starting material; to determine the appropriate culture medium for the multiplication of blackberry in vitro culture, using different growth hormones. The work was carried out in the BITRO PLANTAS laboratory in the Cevallos canton, Province of Tungurahua, the extraction of the vegetative material was in the same place. It was developed with the use of growth hormones such as (auxin, cytokinin, gibberellin) and the combination of these with doses of 2m/L, 4ml/L, 1ml/L in a (DBCA) design. Regarding the number of contaminated explants (NEC), it was recorded 30 days before entering the multiplication phase. The treatment that stood out was T5 (gibberellin plus the auxin combination), the variables recorded were the length of the shoots (BL) at 70 days. In the variable of the number of shoots per explant (NBE) treatment that exceeded the T1 (cytokinin) at 70 days. The variable in the number of leaves per explant (NHE), the treatment that stood out is T5 (gibberellin in combination with auxin wing) at 70 days that the data was taken, being that T1 and T5 have to be applied in the different in vitro propagations for the formation of quality plants. |
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