Identificación de consorcios bacterianos y grupos funcionales asociados a la rizosfera de la papa (Solanum tuberosum), var. Leona Blanca en el piso altitudinal de 3800 msnm. Cotopaxi, 2021
The purpose of this research study was to identify bacterial consortia and determine the functional groups associated with the rhizosphere of the potato crop, super chola variety, at an altitudinal level of 3800 meters above sea level in the community of Cuturuví Chico in the province of Cotopaxi. B...
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| Format: | bachelorThesis |
| Jezik: | spa |
| Izdano: |
2021
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| Teme: | |
| Online pristup: | http://repositorio.utc.edu.ec/handle/27000/8262 |
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Dodaj oznaku | Sažetak: | The purpose of this research study was to identify bacterial consortia and determine the functional groups associated with the rhizosphere of the potato crop, super chola variety, at an altitudinal level of 3800 meters above sea level in the community of Cuturuví Chico in the province of Cotopaxi. Bacterial identification was performed through the nanopore sequencing method with MinION device, with amplification of the 16s genes of the sample and preparation of DNA libraries for sequencing using the barcode kit for subsequent analysis of the resulting readings in the online 16S bioinformatics flow in EPI2ME. For the analysis of the different functional groups, the methodology of (Bernal, Gustavo, 2005) was used as a methodological reference for the determination of nitrogen-fixing bacteria, nitrogen-solubilizing bacteria, fungi, actinomycete, cellulolytic bacteria, pseudomonas. Of the functional groups mentioned, five repetitions were carried out in soil and root plus a blind control where were obtained the number of colonies*gr-1 and the (Colony-forming units) CFU*gr-1 of each one. In addition, a physical-chemical analysis was performed to determine the soil composition, which was sent to the ‘INIAP’ laboratory. From the data obtained in the research, Enterobacteriaceae was identified as the dominant families in the soil sample with 75,596 cumulative readings, these are characterized by being phosphorus solubilizers followed by Xanthomonadaceae with 7,042 cumulate readings, which are characterized by having nitrogen-fixing bacteria. The most dominant genus is Raoultella with 4,468 cumulative readings, followed by Rhodanobacter with 3,146 cumulative readings. The most dominant species was Raoultella ornithinolytica with 2,993 cumulative readings, followed by Gemmatimonas phototrophica with 1,803 cumulative readings, which could be related to the high nitrogen and phosphorus content obtained in the soil analysis with 48 ppm of nitrogen (N) and 52 ppm of phosphorus (P), respectively. |
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