Aislamiento y estudio de la resistencia a los antibacterianos en cepas de Escherichia coli en aislados de deyecciones en pollos broiler de los criaderos de Poaló del cantón Latacunga.

The study of the sensitivity of bacteria to antibiotics has become an important tool in recent years; it is essential to determine their resistance and evaluate the effectiveness of the treatments that will be used to combat them. This research project assumed the objective of analyzing the resistance to antibacterial (AMR) of the bacterium Escherichia Coli from broiler chickens from Poaló hatcheries. For the research work 50 samples were collected in total, of which 40 were sewage and 10 were taken directly from the droppings present in the soil, were marked and preserved in Stuart means of transport, the ISO 16654: 2001 method was used, in the enrichment process soybean broth with Trypticasein (TSB) was used in test tubes, was inoculated, homogenized and incubated, for selective isolation, MacConkey agar was used with Sorbitol, this was inoculated with the samples previously enriched with the techniques of aseptic transfer and culture in stretch marks, in the process of purification of colonies the samples that met the coloration requirements were selected which is a characteristic rose, caused by the fermentation of lactose, which is associated with enterobacteria, of 50 samples 11 belonging to chickens and 5 floor met the requirements and nutritional agar was used, in this the same planting techniques were used as in the previous agar to inoculate it, with the colonies obtained the Gram staining procedure was done, through the observation with microscope the presence of gram-negative bacteria was confirmed, Microgen biochemical tests were performed, and its Microgen ID software revealed that 5 of the samples from chickens and 2 from the floor had the bacterium Escherichia coli, with these samples the sensitivity tests could be performed, with the use of Mueller Hinton agar and the Bauer & Kirby technique. The results obtained showed colony growth in the inhibition halos, being an indication of resistant populations of E. coli originating from the samples studied: Amoxicillin 100% (resistant), Cephalexin 57% (resistant), 43% (intermediate), Ciprofloxacin 43% (resistant), 14% (Intermediate), 43% (Sensitive), Enrofloxacin 14% (resistant), 72% (intermediate), 14% (sensitive), Florfenicol 86% (resistant), 14% (Sensitive), Fosfomycin 57% (resistant), 14% (intermediate), 29 % (sensitive), Gentamicin 43 % resistant 28% (intermediate), 29% (sensitive), Norfloxacin 29% (resistant) 71% (Intermediate), Penicillin G 100% (resistant) Sulf+Trimetroprim 86 % (resistant), 14% (intermediate),Tetracycline 100% (resistant).

• 1. Verfasser: Juma Carcelén, Erick Sebastian (author)
• Format: bachelorThesis
• Sprache: spa
• Veröffentlicht: 2022
• Schlagworte: RESISTENCIA A LOS ANTIBIÓTICOS; ESCHERICHIA COLI; POLLOS BROILER; VETERINARIA
• Online Zugang: http://repositorio.utc.edu.ec/handle/27000/9652