Identificación de comunidades bacterianas y grupos funcionales asociados a la rizosfera de la papa (Solanum tuberosum). Var super chola en el piso altitudinal de 3100 msnm, Cuturivi, Cotopaxi 2022.

This research aimed to identify bacterial communities and determine the functional groups associated with the rhizosphere of the potato in the “Super Chola” variety (solanum tuberosum) at 3100 masl to determine the different functional groups. The methodology of (Bernal, 2015) was used in the locality of Cuturivi, Cotopaxi Province, where culture media are used such as Nutrient Agar (Total Microbiota), Rose Bengal (Total Population of Fungi), Ramos Callao Agar (Phosphorus solubilizers), King's B (Pseudomonas), Soil Extract Agar (Cellulitic Bacteria), Watanabe (Nitrogen Fixers) and Casein Agar (Actinomycetes). Bacterial identification was performed by sequencing 16S rRNA in soil using third-generation sequencing with nanopores (Oxford Nanopore Technologies). Additionally, a physicochemical analysis was carried out at the INIAP laboratory to know the state of soil composition. From the data, it was obtained that in this altitudinal tier, the genre with the most significant presence is Arenimonas with 2,530 accumulated readings, followed by Lactobacillus with 860 readings. These genres are related to nitrogen fixation in the soil, which is consistent with the physical analysis where values of 150 ppm of nitrogen were obtained, considered high. Five repetitions were established in soil and root plus a blind control for each group, where the number of colonies *gr-1 and CFU *gr -1 was established. The functional group with the highest number of colonies was nitrogen-fixing bacteria with 1.18*108 colonies*gr-1 in the soil, and 1.06*108 colonies*gr-1 in the root, followed by phosphorus-solubilizing bacteria with 2, 08*1012 cfu*gr-1, in soil and root with total microbiota with 9.16*1011 cfu*gr-1 respectively. So, there is also the presence in soil and root of the other groups under study. There is a relationship between the bacterial communities present with the functional groups of greater presence and the results of the levels of elements that the potato rhizosphere has.

• Main Author: Armas Ochoa, Galo Sebastian (author)
• Format: bachelorThesis
• Language: spa
• Published: 2022
• Subjects: COMUNIDADES BACTERIANAS; GRUPOS FUNCIONALES; METAGENÓMICOS; AGRONÓMICA
• Online Access: http://repositorio.utc.edu.ec/handle/27000/8777