Métodos de conservación de Beauveria spp. En condiciones de laboratorio Latacunga 2023.
The objective of this research project is to determine a method that preserves entomopathogenic fungal strains in the long term under laboratory conditions, allowing to maintain their viability and conidial production. The research was carried out in the microbiology laboratory of the Technical Univ...
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| 格式: | bachelorThesis |
| 语言: | spa |
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2023
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| 主题: | |
| 在线阅读: | http://repositorio.utc.edu.ec/handle/27000/10971 |
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添加标签 | 总结: | The objective of this research project is to determine a method that preserves entomopathogenic fungal strains in the long term under laboratory conditions, allowing to maintain their viability and conidial production. The research was carried out in the microbiology laboratory of the Technical University of Cotopaxi as a sign that this type of research can be carried out in the institution. Mycelium discs with agar were stored in three conservation methods: mineral oil (BbA), sterile water (Bb), deep freezing -70°+ glycerol solution (BbG) and a control without any substance (BbT) for 30 days. Once the time elapsed, viability tests were carried out through mycelial growth and conidia production. For mycelial growth, the growth diameter was determined on the 8th day of development in a petri dish, in this case the distilled water method and the control treatment had a short difference in growth, being the only ones that obtained results. A completely randomized design was used with 3 repetitions whose experimental units consisted of 3 disks with mycelium and agar of the Beauveria spp. fungus. the control treatment was a strain of the fungus that was not subjected to conservation, significant changes were observed in the mineral oil method since 50% of the samples could not be preserved, on the other hand the -70° deep-freezing method, the discs were they detached and the preservative substance (glycerol) was impregnated in the disc preventing the spread of the fungus. The most effective conservation method was sterile water since it managed to obtain a growth of mycelium of 7.4 cm in diameter compared to the control treatment. In the same way, the sterile water conservation method presented a concentration of conidia of 1.3 x106 compared to the control treatment with a value of 1.08x106. The isolates with the mineral oil and deep-freezing methods at -70° failed to develop mycelial growth or conidia production. The selection of conservation methods facilitates the development of effective protocols so that each entomopathogenic fungus can ensure its purity, stability, growth and viability. |
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