Evaluación del índice de daño ocasionado por Radopholus similis y el efecto biocontrolador de las PGPRs cómo agente supresor en plántulas de banano cultivar Williams

The use of Plant Growth Promoting Rhizobacteria (PGPRs), induce several positive contributions to the control of phytopathogens, promote soil recovery and conservation, increase soil fertility and productivity, and also encourage intracellular growth (iPGPR). While the continuous use of nematicides...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
1. Verfasser: Valarezo Padilla, Flavio Cesar (author)
Format: bachelorThesis
Sprache:spa
Veröffentlicht: 2022
Schlagworte:
Online Zugang:https://repositorio.uteq.edu.ec/handle/43000/6661
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The use of Plant Growth Promoting Rhizobacteria (PGPRs), induce several positive contributions to the control of phytopathogens, promote soil recovery and conservation, increase soil fertility and productivity, and also encourage intracellular growth (iPGPR). While the continuous use of nematicides causes alterations in the metabolism of the nematode, inferring variations to the following phylogeny, it also affects the microbiota. The main objective of this research is to evaluate the rate of damage caused by Radopholus similis and the biocontrol effect of PGPRs as a suppressive agent in seedlings of banana cultivar Williams'. Given the study, after the acclimatization of the seedlings, R. similis was applied with initial populations between 10,000 - 15,000, after 30 days, 4 repopulations were carried out every 7 days with a density of 8,000 - 10,000. The Acinobacter calcoaceticus strains (BMR 2/12), Serratia marcesns (PM 3/8), Pseudomonas Protegens (CHA0) and Enterobacter asburiae (PM 3/14); were prepared in different bioformulates, to be applied 30 days after the inoculation of R. similis. As a result, bacterial growth in suitable bioformulates presents a higher cell concentration of 9.70E+09 CFU/ul. The interaction of the endophytic bacteria shows high loads of CFU/ul starting with 3.00E+09 and ending with 2.50E+09, demonstrating constant cell growth, as well as its cell stability on the rhizosphere that allows loads of 1.26E+10 CFU. /ul. The bioactive compounds produced by the PGPRs bacteria affect the presence of R. similis, limiting its mobility and reproduction; exhibiting damage rates of up to 12.28% and minimum population densities of 1,000/100 root grams. The ability to reduce the viability of the R. similis nematode and the permanence of the bacteria (iPGPR or ePGPR) encourages the use of alternatives of ecological origin for biofertilization, biostimulation and biocontrol of banana crops.