Obtención de extractos acuosos con actividad celulolítica y ligninolitica a partir de la colonización del hongo Pleurotus ostreatus en sustrato pseudotallo de plátano

The use of enzymes for industrial or animal feed uses as a development of biotechnology, presenting a tool for microorganisms to obtain metabolites of interest at both the industrial and field level. Fiber is one of the most important food components, as well as the most problematic, since being present in most of the raw materials of the balanced feed constitutes a limitation in the exploitation due to the low tolerance of Monogastric species to fiber levels in the diet. The extraction of aqueous extracts with enzymatic activity from banana pseudotallo substrate digested by the fungus Pleurotus osteatus was carried out, for which a complete randomized experimental design was established, where the combined effect of the pH of the extraction buffer solution (4.0, 4.8, 6.0 and 7.0) and the effect of the extraction temperature (40, 50 and 60 ° C), thus obtaining 12 treatments. The results showed that the enzymatic extracts act within the optimum pH reaction ranges, so that the pH effect of the buffer solution was significant as it increased, obtaining as the ideal pH the buffer 7.0, which allowed to obtain better characteristics Of the aqueous extracts. The extraction temperature significantly affected the characteristics of the final enzymatic extract, achieving a greater enzymatic activity within the range of 40 ° to 50 ° C. The enzymatic activity demonstrated that the enzymes obtained act within high pH and temperature ranges of 7.0 and temperature of 50 ° C with a positive effect on enzymatic extraction.

• 1. autor: Fuentes Vera, Erika Jessenia (author)
• Format: bachelorThesis
• Język: spa
• Wydane: 2017
• Hasła przedmiotowe: buffer; Temperatura; subproducto; fibra
• Dostęp online: http://repositorio.uteq.edu.ec/handle/43000/2072