Obtención y evaluación de extractos enzimáticos con actividad celulítica y lignolítica a partir del hongo Pleurotus ostreatus cultivados en tuza de maíz.

Fibrolytic enzymes are related to food, textiles, paper, as well as are used for research purposes to improve the digestibility of fiber from agricultural and industrial by-products through biological processes where the addition of fibrolytic enzymes is presented As an alternative for the use of nutrients present in these products. The objective of this study was to obtain enzymatic extracts with cellulite and lignolytic activity from the Pleurotus ostreatus fungus cultivated in maize tui, for which strains of this fibrolytic fungus were cultivated in maize tuna until its development and that degraded the substrate, From which the enzymes were extracted by a protocol where two extraction parameters were evaluated: pH (4.0, 4.8, 6.0 and 7.0) and temperature (40, 50 and 60 ° C). A complete randomized experimental design was used to evaluate 12 treatments resulting from the combination of buffer pH and extraction temperature. The enzymatic extracts obtained were evaluated to determine their pH, temperature and absorbance at 470 nm by spectrophotometry. For pH evaluation, T9 (pH 6.0 + 60 ° C) and T10 (pH 7.0 + 40 ° C) obtained the highest pH values with 5.35 and 5.29 respectively; Whereas in the temperature of the enzymatic extracts the treatments that presented better results were T2 (pH 4.0 + 50 ° C), T4 (4.80 + 40 ° C) and T6 (pH 4.8 + 60 ° C) with 24.8; 25.0 and 24.5 ° C respectively. On the other hand, the highest enzymatic activity was obtained with T9 (pH 6.0 + 60 ° C) and T10 (pH 7.0 + 40 ° C) treatments with absorbance values of 0.903 and 0.889 respectively. It was determined that at higher extraction pH the characteristics of the enzymes obtained were higher, as well as the enzymatic activity, however the extraction temperature had no effect.

• 1. Verfasser: Chávez Véliz, Jacobo Daniel (author)
• Format: bachelorThesis
• Sprache: spa
• Veröffentlicht: 2017
• Schlagworte: pH; Temperatura; espectrofotometría; celulasa
• Online Zugang: http://repositorio.uteq.edu.ec/handle/43000/2071