Selección de microorganismos con capacidad de biorremedación provenientes de aguas residuales del río Quevedo, provincia de Los Ríos, año 2020

Water is one of the most important constituents for life, for this reason it is essential to preserve both physical, chemical and microbiological quality, in order to ensure that its distribution does not contain the presence of pathogenic contaminants that can proliferate water-borne diseases. Microorganisms that are efficient for this type of situation have been reported as an option to solve water contamination problems. They are able to use the polluting compounds present in the waters as a source of carbon and energy for their growth and metabolism. The present study aims to determine the microbial load of the Quevedo river flow, for which five samples were taken along the river, microorganisms were also isolated and selected to verify tolerance to Nitrobenzene and finally the degree of tolerance of said substance under in vitro conditions. To carry out this project, the following methodology was used, adaptation to Nitrobenzene, morphological characterization, biochemical characterization within it, fluorescence, catalase test and Gram stain and finally the progressive growth of the bacteria. After applying the methodology, the results were: The microbial load of the Quevedo river flow is high, it has abundant wastewater discharges and a large volume, which causes the river's self-regeneration capacity to be exceeded and its water deteriorates due to the increase in pathogens for humans and aquatic life. The microorganisms with tolerance to Nitrobenzene isolated and selected in the waters of the Quevedo River are indicators of the existence of elements that are surviving the discharges of residues with nitrobenzene content, they possess metabolic capacities to tolerate hydrocarbons, assimilate and degrade them. The highest tolerance of bacteria in the presence of Nitrobenzene in in vitro conditions was with Strain VI8 since the growth curve of the culture showed a population increase in the hours and concentrations exposed, possibly this strain can be used by other microorganisms or benefit other strains by removal of toxic hydrocarbon compounds. Keywords Nitrobenzene, pathogenic pollutants, water pollution, water transmission.

• Hlavní autor: Bustamante Sandoya, Génesis Fernanda (author)
• Médium: masterThesis
• Jazyk: spa
• Vydáno: 2020
• Témata: Nitrobenceno; Contaminantes patógenos; Contaminación hídrica; Transmisión hídrica
• On-line přístup: https://repositorio.uteq.edu.ec/handle/43000/6390