"Biodegradación de polietileno mediante el uso de microorganismos"
Polyethylene is a polymer that causes global environmental problems through degradation and deterioration that leads to its accumulation in the environment and, therefore, creates serious environmental problems. An attempt has been made to cover the biodegradation mechanism, the various bacterial an...
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| Формат: | bachelorThesis |
| Мова: | spa |
| Опубліковано: |
2020
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| Предмети: | |
| Онлайн доступ: | http://repositorio.uteq.edu.ec/handle/43000/5938 |
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| Резюме: | Polyethylene is a polymer that causes global environmental problems through degradation and deterioration that leads to its accumulation in the environment and, therefore, creates serious environmental problems. An attempt has been made to cover the biodegradation mechanism, the various bacterial and fungal organisms that have been reported for them, the methods adopted for the studies and the different characterization techniques followed to measure the degree of degradation. Different strains from the UTEQ molecular biology and microbiology laboratory were chosen for the respective work. Biochemical tests were performed (degradation of urea, phosphorus and potassium), absorbance, biomass, CFU, pH, polyethylene degradation were measured and absorbance was correlated with CFU. Among the results, the VI8 strain had the highest absorbance at 0.21 at 10 days, the bacterial strain that generated the highest cell biomass was RR4, which generated averages of 0.06 gr / ml at 10 days, the cell content of bacteria in VI8 reach averages of 2.1x10 ^ 6, the Hydrogen Potential in the PN7 strain was maintained at a pH of 7.2 to 6.6 during the 30 days, in the degradation of polyethylene, the strain with the greatest degradation of polyethylene at 300 mg was the strain VI8 reduced 32 and 38 mg at 15 and 30 days respectively. It was concluded that the rate of degradation depends on multiple biochemical factors such as the production of organic and inorganic acids that break down the plastic molecules into simpler fractions that can be assimilated by other types of microorganisms. Keywords: Contamination, D.O. Preservation, Urease, Phosphatase |
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