Quintana Álava, Edgar Mesías. (2020). Aceites esenciales de Tagetes spp. (Flor de muerto) y su efecto bactericida a Ralstonia Solanacearum en condiciones in vitro. Quevedo. UTEQ. 100p.
In the present investigation, the essential oil obtained from flowers and leaves was evaluated separately from two species of Tagetes spp. coming from the coastal region (floor 1) and Sierra (floor 2). As inhibitors of the growth of R. solanacearum at the in x vitro level, s and used a completely ra...
Wedi'i Gadw mewn:
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| Fformat: | masterThesis |
| Iaith: | spa |
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2020
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| Mynediad Ar-lein: | https://repositorio.uteq.edu.ec/handle/43000/6294 |
| Tagiau: |
Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!
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Ychwanegu Tag | Crynodeb: | In the present investigation, the essential oil obtained from flowers and leaves was evaluated separately from two species of Tagetes spp. coming from the coastal region (floor 1) and Sierra (floor 2). As inhibitors of the growth of R. solanacearum at the in x vitro level, s and used a completely randomized design 4 x 5. The essential oils of leaves and flowers were obtained by means of hydrodestilation, the extraction performance of the essential oils was evaluated, obtaining that the highest yields were obtained from the leaves of both species evaluated: with 0.0178 and 0.01576 % for plant 1 and plant 2, respectively, while treatments corresponding to flowers and fragments of calyx showed yields of 0.00684 and 0.00316% for plant 2 and plant 1. Qualitative tests gave as a result: Gram (-), catalase (+) and without showing f uorescence under UV at 360 Nm, making compatible with the characteristics described in the literature for this bacterium. The growth kinetics of R. solanacearum in King B culture medium was determined as a starting point to determine the maximum incubation time to perform the inhibition tests, at the same time another kinetics was performed with the addition of the solvent of the essential oils (70% ethanol -3% V / V). It was determined that the stationary phase is reached at 12 hours of incubation. Evaluation of two concentrations (2 and 3%) essential oil against the pathogen not been sufficient to meet the minimum inhibitory concentration -MIC due to the presence of turbidity in all ensa yos. The sub - cultures performed showed that there existed within the concentrations evaluated Concentration Minimum Bactericidal-MBC, however l os results indicate that all concentrations exerted some inhibition of bacterial growth emphasizing the essential oil obtained from flowers of the plant 1 with percent 90.13% inhibition. Key words: Hydrodestilation , Growth kinetics, Essential oil, Minimum inhibitory concentration - MIC, Minimum Bactericidal Concentration-MBC. |
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