Phytochemical study and evaluation of cytotoxic and genotoxicproperties of extracts from Clusia latipes leaves
Some species of the Clusia genus have been shown to have important biomedical properties, includingthe ability to inhibit tumor growth in vitro and the usefulness for skin care. In this study, we exam-ined the cytotoxic effect of hexane, ethyl acetate and methanol extracts from Clusia latipes Planch...
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| Άλλοι συγγραφείς: | , |
| Μορφή: | article |
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2015
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| Διαθέσιμο Online: | http://dspace.utpl.edu.ec/handle/123456789/18896 |
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Προσθήκη ετικέτας | _version_ | 1839346723047079936 |
|---|---|
| author | Bailon Moscoso, N. |
| author2 | Silva, R. Romero Benavides, J. |
| author2_role | author author |
| author_facet | Bailon Moscoso, N. Silva, R. Romero Benavides, J. |
| author_role | author |
| collection | Repositorio Universidad Técnica Particular de Loja |
| dc.creator.none.fl_str_mv | Bailon Moscoso, N. Silva, R. Romero Benavides, J. |
| dc.date.none.fl_str_mv | 10/12/2015 2015-08-01 2016-01-01 2017-06-16T22:02:34Z 2017-06-16T22:02:34Z |
| dc.identifier.none.fl_str_mv | doi:10.1016/j.bjp.2015.08.014 0102695X doi:10.1016/j.bjp.2015.08.014 http://dspace.utpl.edu.ec/handle/123456789/18896 |
| dc.language.none.fl_str_mv | Inglés |
| dc.publisher.none.fl_str_mv | Brazilian Journal of Pharmacognsosy |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio Universidad Técnica Particular de Loja instname:Universidad Técnica Particular de Loja instacron:UTPL |
| dc.subject.none.fl_str_mv | Anticancer phytometabolites Clusia latipes Cytotoxicity Genotoxicity Comet assay |
| dc.title.none.fl_str_mv | Phytochemical study and evaluation of cytotoxic and genotoxicproperties of extracts from Clusia latipes leaves |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| description | Some species of the Clusia genus have been shown to have important biomedical properties, includingthe ability to inhibit tumor growth in vitro and the usefulness for skin care. In this study, we exam-ined the cytotoxic effect of hexane, ethyl acetate and methanol extracts from Clusia latipes Planch. &Triana, Clusiaceae, leaves on survival of human prostate cancer cells (PC-3), colon cancer cells (RKO),astrocytoma cells (D-384), and breast cancer cells (MCF-7). The ethyl acetate extract displayed the mostsubstantial cytotoxic effect. However, using a Comet assay, we observed that the hexane extract induceda genotoxic effect (DNA damage) on human lymphocytes in an in vitro model. Chromatographic purifi-cation of the C. latipes hexane extract led to the isolation and identification of friedelin, friedolan-3-ol,and hesperidin as active cytotoxic compounds in hexane extract, while -amyrine was identified as anactive cytotoxic compound in the ethyl acetate extract of C. latipes, thereby supporting further stud-ies of the molecular mechanisms underlying the effect of these secondary metabolites on cancer cellsurvival. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UTPL_1e2e7e76917f29992c91bc37abbcd3a1 |
| identifier_str_mv | doi:10.1016/j.bjp.2015.08.014 0102695X |
| instacron_str | UTPL |
| institution | UTPL |
| instname_str | Universidad Técnica Particular de Loja |
| language_invalid_str_mv | Inglés |
| network_acronym_str | UTPL |
| network_name_str | Repositorio Universidad Técnica Particular de Loja |
| oai_identifier_str | oai:dspace.utpl.edu.ec:123456789/18896 |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Brazilian Journal of Pharmacognsosy |
| reponame_str | Repositorio Universidad Técnica Particular de Loja |
| repository.mail.fl_str_mv | . |
| repository.name.fl_str_mv | Repositorio Universidad Técnica Particular de Loja - Universidad Técnica Particular de Loja |
| repository_id_str | 1227 |
| spelling | Phytochemical study and evaluation of cytotoxic and genotoxicproperties of extracts from Clusia latipes leavesBailon Moscoso, N.Silva, R.Romero Benavides, J.AnticancerphytometabolitesClusia latipesCytotoxicityGenotoxicityComet assaySome species of the Clusia genus have been shown to have important biomedical properties, includingthe ability to inhibit tumor growth in vitro and the usefulness for skin care. In this study, we exam-ined the cytotoxic effect of hexane, ethyl acetate and methanol extracts from Clusia latipes Planch. &Triana, Clusiaceae, leaves on survival of human prostate cancer cells (PC-3), colon cancer cells (RKO),astrocytoma cells (D-384), and breast cancer cells (MCF-7). The ethyl acetate extract displayed the mostsubstantial cytotoxic effect. However, using a Comet assay, we observed that the hexane extract induceda genotoxic effect (DNA damage) on human lymphocytes in an in vitro model. Chromatographic purifi-cation of the C. latipes hexane extract led to the isolation and identification of friedelin, friedolan-3-ol,and hesperidin as active cytotoxic compounds in hexane extract, while -amyrine was identified as anactive cytotoxic compound in the ethyl acetate extract of C. latipes, thereby supporting further stud-ies of the molecular mechanisms underlying the effect of these secondary metabolites on cancer cellsurvival.Brazilian Journal of Pharmacognsosy2017-06-16T22:02:34Z2015-08-012017-06-16T22:02:34Z2016-01-0110/12/2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articledoi:10.1016/j.bjp.2015.08.0140102695Xdoi:10.1016/j.bjp.2015.08.014http://dspace.utpl.edu.ec/handle/123456789/18896Inglésinfo:eu-repo/semantics/openAccessreponame:Repositorio Universidad Técnica Particular de Lojainstname:Universidad Técnica Particular de Lojainstacron:UTPL2017-06-16T22:02:34Zoai:dspace.utpl.edu.ec:123456789/18896Institucionalhttps://dspace.utpl.edu.ec/Institución privadahttps://www.utpl.edu.ec/https://dspace.utpl.edu.ec/oai.Ecuador...opendoar:12272017-06-16T22:02:34Repositorio Universidad Técnica Particular de Loja - Universidad Técnica Particular de Lojafalse |
| spellingShingle | Phytochemical study and evaluation of cytotoxic and genotoxicproperties of extracts from Clusia latipes leaves Bailon Moscoso, N. Anticancer phytometabolites Clusia latipes Cytotoxicity Genotoxicity Comet assay |
| status_str | publishedVersion |
| title | Phytochemical study and evaluation of cytotoxic and genotoxicproperties of extracts from Clusia latipes leaves |
| title_full | Phytochemical study and evaluation of cytotoxic and genotoxicproperties of extracts from Clusia latipes leaves |
| title_fullStr | Phytochemical study and evaluation of cytotoxic and genotoxicproperties of extracts from Clusia latipes leaves |
| title_full_unstemmed | Phytochemical study and evaluation of cytotoxic and genotoxicproperties of extracts from Clusia latipes leaves |
| title_short | Phytochemical study and evaluation of cytotoxic and genotoxicproperties of extracts from Clusia latipes leaves |
| title_sort | Phytochemical study and evaluation of cytotoxic and genotoxicproperties of extracts from Clusia latipes leaves |
| topic | Anticancer phytometabolites Clusia latipes Cytotoxicity Genotoxicity Comet assay |
| url | http://dspace.utpl.edu.ec/handle/123456789/18896 |