Detection and molecular characterization of ?-lactamase genes in clinical isolates of Gram-negative bacteria in Southern Ecuador

This work performed a phenotypic and genotypic characterization of 79 clinical isolates of Enterobacteriaceae and Pseudomonadaceae collected in hospitals of Southern Ecuadorin 2013. Our results showed a high incidence of ?-lactamases and ESBLs with blaTEM and blaCTX-M as the prevalent genes, respectively. By direct sequencing of PCR amplicons, the different ?-lactamases and variants of the genes were also distinguished. Our results revealed a predominance of TEM-1 ?-lactamase and the presence of different CTX-M variants with a prevalence of CTX-M-15. Two infrequent CTX-M variants in South America were also identified. To the best of our knowledge, this is one of the first studies describing the genetic characteristics of ?-lactamases in Ecuador.

• 1. autor: Arevalo Jaramillo, A. (author)
• Kolejni autorzy: Ausili, A. (author), Toledo Barrigas, Z. (author)
• Format: article
• Wydane: 2016
• Hasła przedmiotowe: ?-Lactam resistance; ?-Lactamase; ESBL; Genotypic characterization
• Dostęp online: http://dx.doi.org/10.1016/j.bjid.2016.07.001; http://dspace.utpl.edu.ec/handle/123456789/18747